Protein Info for Rru_A1856 in Rhodospirillum rubrum S1H

Annotation: (p)ppGpp synthetase I (GTP pyrophosphokinase), SpoT/RelA (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 715 TIGR00691: RelA/SpoT family protein" amino acids 28 to 707 (680 residues), 727.8 bits, see alignment E=6.3e-223 PF13328: HD_4" amino acids 28 to 178 (151 residues), 163.1 bits, see alignment E=1.3e-51 PF01966: HD" amino acids 47 to 146 (100 residues), 33.3 bits, see alignment E=1.6e-11 PF04607: RelA_SpoT" amino acids 238 to 347 (110 residues), 145.4 bits, see alignment E=2.5e-46 PF02824: TGS" amino acids 389 to 447 (59 residues), 75.6 bits, see alignment 7.7e-25 PF19296: RelA_AH_RIS" amino acids 458 to 624 (167 residues), 132 bits, see alignment E=8.7e-42 PF13291: ACT_4" amino acids 639 to 712 (74 residues), 55.9 bits, see alignment E=1.7e-18

Best Hits

KEGG orthology group: K00951, GTP pyrophosphokinase [EC: 2.7.6.5] (inferred from 100% identity to rru:Rru_A1856)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RT89 at UniProt or InterPro

Protein Sequence (715 amino acids)

>Rru_A1856 (p)ppGpp synthetase I (GTP pyrophosphokinase), SpoT/RelA (NCBI) (Rhodospirillum rubrum S1H)
MAMMRQFELVERVKAYDPNANEDLLNRAYVYAMKMHGSQLRASGDPYFSHPIDVAGILTR
YKLDCASIITALLHDTIEDTEATLEDVRQLFGNDVARLVDGVTKLTRIQISSDHAKQAEN
FRKLLLAMSEDIRVLMVKLADRLHNMRTLHFIRKPEKRARIALETMEIYAPLAERIGMAE
IKEELEDLAFRELYPDAKEGIEARLTFLREQGGTLVPKVIDQLKAALAEHGLTAEITGRE
KTPYSIWQKMKRKNISFEQLSDIMAFRITVENLEQCYQALGIIHASYPMIPDRFKDYIST
PKRNGYQSIHTAVIGPERLRIEIQIRTAEMHSVAELGVAAHWSYKQGSPVMDGRQYRWIR
ELLEILEHTSSPEEFLEHTKLEMFSDQVFCFTPKGDLIGLPSGATPVDFAYAVHTQVGDR
CVSAKVNGRLVPLRTPLHNGDQVEIATSKAQTPSPEWERFVVTGKARAQIRKFVRSQRRD
QYLILGRQLLERAFKAENYEMTEKGLDGVLKKFRADSVEDLIVDVGEGQLPAREVVQAVY
PGLKMVPKLAAVVTLGRTKKPRASDDHSFKIKGMIPGMALHFAKCCHSLPGDRIVGIVTT
GKGITIHTIDCDQLEQFSDEPERWLDLAWDAEADSQVHVGRVNVVVANEPGSLGALTTVI
AKNLGNITNLKITNRQQDFFDMIVDVEVKDVRHLTNIIAALRATSAINYVERARH