Protein Info for Rru_A1815 in Rhodospirillum rubrum S1H

Annotation: Glycolate reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00389: 2-Hacid_dh
  • amino acids 7 to 322 (316 residues), 86.9 bits, E=1.5e-28
• PF02826: 2-Hacid_dh_C
  • amino acids 115 to 291 (177 residues), 181.7 bits, E=1.4e-57
• PF03446: NAD_binding_2
  • amino acids 154 to 241 (88 residues), 25 bits, E=2.6e-09

Best Hits

Swiss-Prot: 44% identical to DHGY_HYPME: Glycerate dehydrogenase from Hyphomicrobium methylovorum

KEGG orthology group: K00015, glyoxylate reductase [EC: 1.1.1.26] (inferred from 100% identity to rru:Rru_A1815)

MetaCyc: 44% identical to hydroxypyruvate reductase subunit (Hyphomicrobium methylovorum GM2)
Hydroxypyruvate reductase. [EC: 1.1.1.81]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81)" in subsystem Glycolate, glyoxylate interconversions or Photorespiration (oxidative C2 cycle) (EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

• photorespiration I (6/9 steps found)
• photorespiration III (6/9 steps found)
• photorespiration II (6/10 steps found)
• L-serine biosynthesis II (1/4 steps found)
• L-arabinose degradation IV (3/8 steps found)
• D-xylose degradation IV (2/7 steps found)
• formaldehyde assimilation I (serine pathway) (6/13 steps found)
• superpathway of pentose and pentitol degradation (9/42 steps found)

KEGG Metabolic Maps

• Glycine, serine and threonine metabolism
• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.26, 1.1.1.79, 1.1.1.81

Use Curated BLAST to search for 1.1.1.26 or 1.1.1.79 or 1.1.1.81

Sequence Analysis Tools

See Q2RTD0 at UniProt or InterPro

Protein Sequence (323 amino acids)

>Rru_A1815 Glycolate reductase (NCBI) (Rhodospirillum rubrum S1H)
MLGKPVVLVTRTLPAAVEERLLGDYDVWLNRDDRPIPPEDLPALARRLGAQAMLVTPTDR
LERAVIEALPNSVAIIASFSVGYEHIDHNAAARRGILVTNTPGVLSDATADIALLLMLGA
ARRASEGERLVRSGYWKGLTPVQLLGRHLHGQRLGILGMGRIGQALAERARPLGLEIHYH
NRTPIAEDAAKGAIFHATVEDLLAVSDVLSLHCPATPLTRKLLNAERLALLPPGAIVVNT
ARGILIDDEALIAALNSGQVFAAGLDVYDNEPDLHPAYRSLPNVFLLPHLGSATIETRTA
MGFLALDNLDAYFGGREPPHRIA