Protein Info for Rru_A1768 in Rhodospirillum rubrum S1H

Annotation: Protein-L-isoaspartate(D-aspartate) O-methyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 219 TIGR00080: protein-L-isoaspartate O-methyltransferase" amino acids 2 to 210 (209 residues), 211 bits, see alignment E=9.2e-67 PF01135: PCMT" amino acids 7 to 210 (204 residues), 203.9 bits, see alignment E=2.7e-64 PF00398: RrnaAD" amino acids 69 to 119 (51 residues), 25.4 bits, see alignment E=7.2e-10

Best Hits

Swiss-Prot: 100% identical to PIMT_RHORT: Protein-L-isoaspartate O-methyltransferase (pcm) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K00573, protein-L-isoaspartate(D-aspartate) O-methyltransferase [EC: 2.1.1.77] (inferred from 100% identity to rru:Rru_A1768)

MetaCyc: 49% identical to protein-L-isoaspartate O-methyltransferase (Escherichia coli K-12 substr. MG1655)
Protein-L-isoaspartate(D-aspartate) O-methyltransferase. [EC: 2.1.1.77]

Predicted SEED Role

"Protein-L-isoaspartate O-methyltransferase (EC 2.1.1.77)" in subsystem Ton and Tol transport systems (EC 2.1.1.77)

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.77

Use Curated BLAST to search for 2.1.1.77

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RTH7 at UniProt or InterPro

Protein Sequence (219 amino acids)

>Rru_A1768 Protein-L-isoaspartate(D-aspartate) O-methyltransferase (NCBI) (Rhodospirillum rubrum S1H)
MSVPSRKIRLIMELRQNGVSATPVLAAIERVPRDAFVSAPFSDQAYENTALPIGCGQTIS
QPLVVGLMTQALDLNDRHKVLEIGTGSGYQTAVLARLCRRVYTIERHGALLREAEARLTA
LGLHRTVVTREGDGGRGWPEQAPFERILVTAAALDIPKVLVAQLAIGGVMVLPVGKESGA
QEVVRVRRTAEDALVTERLFPVRFVPLVDGLPPRDAPGA