Protein Info for Rru_A1680 in Rhodospirillum rubrum S1H

Annotation: Two Component, Sigma54 Specific, Transcriptional Regulator, Fis family (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 PF00072: Response_reg" amino acids 5 to 116 (112 residues), 97.6 bits, see alignment E=1.5e-31 PF00158: Sigma54_activat" amino acids 142 to 309 (168 residues), 193.5 bits, see alignment E=6.8e-61 PF14532: Sigma54_activ_2" amino acids 143 to 314 (172 residues), 72.3 bits, see alignment E=1.5e-23 PF00004: AAA" amino acids 166 to 301 (136 residues), 21.5 bits, see alignment E=8e-08 PF07728: AAA_5" amino acids 166 to 285 (120 residues), 31.9 bits, see alignment E=3.8e-11 PF02954: HTH_8" amino acids 416 to 454 (39 residues), 39.5 bits, see alignment 1.1e-13

Best Hits

Swiss-Prot: 58% identical to NTRX_AZOC5: Nitrogen assimilation regulatory protein NtrX (ntrX) from Azorhizobium caulinodans (strain ATCC 43989 / DSM 5975 / JCM 20966 / NBRC 14845 / NCIMB 13405 / ORS 571)

KEGG orthology group: K13599, two-component system, NtrC family, nitrogen regulation response regulator NtrX (inferred from 100% identity to rru:Rru_A1680)

Predicted SEED Role

"Nitrogen regulation protein NtrX"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RTR5 at UniProt or InterPro

Protein Sequence (466 amino acids)

>Rru_A1680 Two Component, Sigma54 Specific, Transcriptional Regulator, Fis family (NCBI) (Rhodospirillum rubrum S1H)
MAHDILIVDDEEDIRMMLAGILEDEGYDTRVAVDADSCFKAIDERTPAVVILDIWMQGSR
LDGLQVLEELARDHPYLPVVMISGHGTIETAVSAIKQGAFDFIEKPFKADRLLLIIQRAI
ETAQLKLENAELRMRAGPETQMIGDSALIGHVAQTIAKVAPTGSRVLISGPPGTGKEVVA
RMIHAHSPRAQNRLVVVNCAAMHPDRMEIELLGAEPGSGDGADSYRKIGLFEQADGGTLL
LDEVADMPLETQGKIVRILQDQSFERVGGGRRIRVDVRVIASTNRDLAQEIALGRFREDL
YYRLNVVPIRLPALRERREDITPLALHFMDRAASSAGLPPRPFGEDALAALQSYDWPGNV
RQLRNVVDWLLIMAPGEAREAIRADMLPAEIGSVTPDSLRLDRSGEIMTLPLREAREMFE
RDYLIAQVTRFGGNVSKTAEFVGMERSALHRKLKSLGVGGSDRQKV