Protein Info for Rru_A1471 in Rhodospirillum rubrum S1H

Annotation: Butyrate-acetoacetate CoA-transferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02428: 3-oxoacid CoA-transferase, B subunit
  • amino acids 4 to 206 (203 residues), 291.3 bits, E=1.6e-91
• PF01144: CoA_trans
  • amino acids 7 to 200 (194 residues), 149.3 bits, E=5.6e-48

Best Hits

Swiss-Prot: 58% identical to CTFB_CLOAB: Butyrate--acetoacetate CoA-transferase subunit B (ctfB) from Clostridium acetobutylicum (strain ATCC 824 / DSM 792 / JCM 1419 / LMG 5710 / VKM B-1787)

KEGG orthology group: K01029, 3-oxoacid CoA-transferase subunit B [EC: 2.8.3.5] (inferred from 100% identity to rru:Rru_A1471)

MetaCyc: 58% identical to butyrate-acetoacetate CoA-transferase, subunit B (Clostridium acetobutylicum ATCC 824)
Acetoacetyl-CoA hydrolase. [EC: 3.1.2.11]; Butyrate--acetoacetate CoA-transferase. [EC: 3.1.2.11, 2.8.3.9]; 2.8.3.9 [EC: 3.1.2.11, 2.8.3.9]

Predicted SEED Role

"Butyrate-acetoacetate CoA-transferase subunit B (EC 2.8.3.9)" in subsystem Acetyl-CoA fermentation to Butyrate or Polyhydroxybutyrate metabolism (EC 2.8.3.9)

MetaCyc Pathways

• ketolysis (3/3 steps found)
• acetoacetate degradation (to acetyl CoA) (2/2 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• pyruvate fermentation to acetone (4/5 steps found)
• isopropanol biosynthesis (engineered) (3/5 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (11/17 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (8/13 steps found)
• L-lysine fermentation to acetate and butanoate (5/10 steps found)
• superpathway of L-lysine degradation (12/43 steps found)

KEGG Metabolic Maps

• Butanoate metabolism
• Synthesis and degradation of ketone bodies
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.8.3.5 or 2.8.3.9 or 3.1.2.11

Sequence Analysis Tools

See Q2RUC3 at UniProt or InterPro

Protein Sequence (219 amino acids)

>Rru_A1471 Butyrate-acetoacetate CoA-transferase (NCBI) (Rhodospirillum rubrum S1H)
MDDKTLIAKRVALELRPGDLANLGIGLPTLVASYLTPGVEVFFQSENGIVGMSPLPDIGF
ADDDLTDAGGSPIGVLPGACAFDSAFSFGLIRGGHLDVTVLGGLQIDERGLLANWMVPGK
MVPGMGGAMDLVSGAKRVIVAMTHTAKGAPKIVKTCTLPLTSVRRVDLVVTELAVIEPTA
KGLLLREVAPGVSVDQVIEATEANLLIAEDLREMPILAA