Protein Info for Rru_A1309 in Rhodospirillum rubrum S1H

Annotation: acetoacetyl-CoA reductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 774 signal peptide" amino acids 1 to 27 (27 residues), see Phobius details PF02737: 3HCDH_N" amino acids 6 to 189 (184 residues), 162.1 bits, see alignment E=3.4e-51 PF03446: NAD_binding_2" amino acids 6 to 52 (47 residues), 21.7 bits, see alignment 4.7e-08 PF00725: 3HCDH" amino acids 192 to 293 (102 residues), 55.3 bits, see alignment E=2e-18 amino acids 346 to 401 (56 residues), 29.9 bits, see alignment 1.7e-10 PF00378: ECH_1" amino acids 472 to 607 (136 residues), 53.2 bits, see alignment E=7.1e-18 PF16113: ECH_2" amino acids 479 to 605 (127 residues), 32.8 bits, see alignment E=1.4e-11

Best Hits

KEGG orthology group: K07516, 3-hydroxyacyl-CoA dehydrogenase [EC: 1.1.1.35] (inferred from 100% identity to rru:Rru_A1309)

Predicted SEED Role

"Enoyl-CoA hydratase (EC 4.2.1.17) / 3,2-trans-enoyl-CoA isomerase (EC 5.3.3.8) / 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)" in subsystem Acetyl-CoA fermentation to Butyrate or Isoleucine degradation or Polyhydroxybutyrate metabolism or Valine degradation or n-Phenylalkanoic acid degradation or Butanol Biosynthesis (EC 1.1.1.35, EC 4.2.1.17, EC 5.3.3.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.35, 4.2.1.17

Use Curated BLAST to search for 1.1.1.35 or 4.2.1.17 or 5.3.3.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RUT5 at UniProt or InterPro

Protein Sequence (774 amino acids)

>Rru_A1309 acetoacetyl-CoA reductase (NCBI) (Rhodospirillum rubrum S1H)
MSEIAKVAVIGAGVMGAGIAAHVANAGLPVLLLDIVAKDSEDRSAIAAKAIERLQKSEPS
AFMGKAAAKRVTPGNIEDDLDKLADCDWIVEAILERADLKQDLYRRVEAVRKDGSVVSSN
TSTIPLSTLTEGLGDRFAEDFLVTHFFNPPRYMRLLEVVAGAQTKPEVVETISRFADHRL
GKGVVACKDRPGFIANRLGVYWLQAAVAEAFDHNLSIEEADAVIGRPMGIPKTGVFGLLD
LVGLDLMPHVNASLAAALPKDDPFQAYNRPLAVVDKMIAEGYTGRKGKGGFYRITRAKGG
KVKEAIDLHSGEWRPERPADPEAIRVAGRDLRALLDHDSPVGRYARAVLTRTLSYAALLV
GDAADDPAAIDDAMRLGYNWKQGPFELIDRLGADWLAAALTAQGDPVPPYLAQAAGRKVY
KVTEGHREILQPDGAYRSHLRPDGVLLLEDIKLVSKPLLKNGSAALWDIGDGVACFEFTS
KMNALDPDILGLLAKSLKLVARQFKALVIYNEGGAFSAGANLGLALFAANIAAWSEIETL
VAEGQKTYRALREAPFPVVGAPSGLALGGGCEILLHCDAVQAHAESYIGLVEVGVGLVPA
WGGCTEMLHRWSTLGQLPKGPMPALSKVFETVSTATVAKSAAEAKELLFLREGDGITMNR
DRLLADAKAKALALVEGYAPPPRREFTLPGAAAKTGLDMAVASVQRQGKATPYDGVVCGA
LADILSGGDTDITVTLTEDALLELERTHFMALARRPETLARIETMLLTGKPLRN