Protein Info for Rru_A1201 in Rhodospirillum rubrum S1H

Annotation: MaoC-like dehydratase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF19315: MC_hydratase
  • amino acids 6 to 317 (312 residues), 65.7 bits, E=6.6e-22
• PF01575: MaoC_dehydratas
  • amino acids 14 to 126 (113 residues), 28.8 bits, E=1.2e-10
• PF13452: FAS1_DH_region
  • amino acids 51 to 140 (90 residues), 22.2 bits, E=2e-08

Best Hits

Swiss-Prot: 66% identical to MCH_CHLAA: Beta-methylmalyl-CoA dehydratase (mch) from Chloroflexus aurantiacus (strain ATCC 29366 / DSM 635 / J-10-fl)

KEGG orthology group: K14449, mesaconyl-CoA hydratase (inferred from 100% identity to rru:Rru_A1201)

MetaCyc: 66% identical to beta-methylmalyl-CoA dehydratase monomer (Chloroflexus aurantiacus)
RXN-8960 [EC: 4.2.1.148]

Predicted SEED Role

"Mesaconyl-CoA hydratase"

MetaCyc Pathways

• methylaspartate cycle (15/19 steps found)
• ethylmalonyl-CoA pathway (8/11 steps found)
• superpathway of the 3-hydroxypropanoate cycle (12/18 steps found)
• glyoxylate assimilation (7/13 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (6/14 steps found)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.2.1.148

Sequence Analysis Tools

See Q2RV43 at UniProt or InterPro

Protein Sequence (353 amino acids)

>Rru_A1201 MaoC-like dehydratase (NCBI) (Rhodospirillum rubrum S1H)
MGKTNPGNFFEDFAPGQQLVHATPRTLTEGDAALYTALYGSRFAVQSSASFAMAIGYPEA
PLDDLLVFHVVFGKTVPDISLNAVANLGYARGRMGVPVYPGDTLRALSRVIGVKENSNGK
TGVVYVNSVGLNQNDEVVVDFIRWVMVQKRDPAHPAPEPEIPDLPDRVAPEDLYLPEGLD
PRGYDPELAGSAHFWEDYAVGERIDHGDGMTIEEAEHMMATRLWQNTAKVHFNQYEQAKG
RFGRRLVYGGHVISLARALSFNGLGNAFRVAAINAGSHCNPTFAGDTIHAWSEVLERADL
PADEGFGALRLRTIATKDRACADFPYRDEQGHIRPEVVLDLDYWVVMPKAEGK