Protein Info for Rru_A1200 in Rhodospirillum rubrum S1H

Annotation: Citrate lyase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03328: HpcH_HpaI
  • amino acids 9 to 223 (215 residues), 153 bits, E=7.6e-49

Best Hits

Swiss-Prot: 51% identical to MCTE_RHOSK: (3S)-malyl-CoA thioesterase (mcl2) from Rhodobacter sphaeroides (strain KD131 / KCTC 12085)

KEGG orthology group: K01644, citrate lyase subunit beta / citryl-CoA lyase [EC: 4.1.3.34 4.1.3.6] (inferred from 100% identity to rru:Rru_A1200)

Predicted SEED Role

"L-malyl-CoA/beta-methylmalyl-CoA lyase (EC 4.1.3.-)" (EC 4.1.3.-)

MetaCyc Pathways

• citrate degradation (2/2 steps found)
• reductive TCA cycle II (8/12 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of plant hormones
• Citrate cycle (TCA cycle)
• Pyruvate metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.-, 4.1.3.6

Use Curated BLAST to search for 4.1.3.- or 4.1.3.34 or 4.1.3.6

Sequence Analysis Tools

See Q2RV44 at UniProt or InterPro

Protein Sequence (303 amino acids)

>Rru_A1200 Citrate lyase (NCBI) (Rhodospirillum rubrum S1H)
MSFSVRPRRSVLYMPGSNTRALEKARTLAADGLILDLEDATAPDAKEDARGHVCAAAQAR
SYGQREVLIRVNGLNTAWGHADLVACATSGCDGVLLPKVESADGVRQAEAILRAAGAPDD
LALWCMMETPRGVLDVAAIAGASPRLAGLVMGTSDLAKDLHCAHTLERLPLITGLGLCLL
AARANGLAILDGVHLDLQDDEGFALSCAQGRELGFDGKTLIHPKTIGAANAAFAPSAEEV
AWSRRIIEAHAEAVREGKGVVLVDGKLVENLHVESARRLVTLDEKIAALGGDLAQDRAMG
SQA