Protein Info for Rru_A1118 in Rhodospirillum rubrum S1H

Annotation: Hydrophobe/amphiphile efflux-1 HAE1 (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1000 1052 transmembrane" amino acids 12 to 32 (21 residues), see Phobius details amino acids 340 to 359 (20 residues), see Phobius details amino acids 366 to 387 (22 residues), see Phobius details amino acids 393 to 413 (21 residues), see Phobius details amino acids 436 to 459 (24 residues), see Phobius details amino acids 471 to 497 (27 residues), see Phobius details amino acids 537 to 555 (19 residues), see Phobius details amino acids 867 to 886 (20 residues), see Phobius details amino acids 892 to 912 (21 residues), see Phobius details amino acids 919 to 943 (25 residues), see Phobius details amino acids 965 to 987 (23 residues), see Phobius details amino acids 998 to 1023 (26 residues), see Phobius details PF00873: ACR_tran" amino acids 1 to 1022 (1022 residues), 1126.5 bits, see alignment E=0 TIGR00915: RND transporter, hydrophobe/amphiphile efflux-1 (HAE1) family" amino acids 1 to 1036 (1036 residues), 1421.6 bits, see alignment E=0

Best Hits

Swiss-Prot: 51% identical to ACRB_ECOLI: Multidrug efflux pump subunit AcrB (acrB) from Escherichia coli (strain K12)

KEGG orthology group: K03296, hydrophobic/amphiphilic exporter-1 (mainly G- bacteria), HAE1 family (inferred from 69% identity to atu:Atu3202)

MetaCyc: 51% identical to multidrug efflux pump RND permease AcrB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-354; TRANS-RXN-355; TRANS-RXN-356; TRANS-RXN-357; TRANS-RXN-359; TRANS-RXN0-592

Predicted SEED Role

"RND efflux system, inner membrane transporter CmeB" in subsystem Multidrug Resistance Efflux Pumps or Multidrug efflux pump in Campylobacter jejuni (CmeABC operon)

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RVC6 at UniProt or InterPro

Protein Sequence (1052 amino acids)

>Rru_A1118 Hydrophobe/amphiphile efflux-1 HAE1 (NCBI) (Rhodospirillum rubrum S1H)
MAQLFIKRPILAWVFAIFISIAGIIALPLLPVAQYPNVAPPQLSLYTTYPGASPQEIYQG
VTRLIEEELNSVDNLIYFESTSDSTGGITINATFQPGTNLQQASVNVQNAIRSVASRLPA
AVTAQGVTVEEVTAGFLMLITLTSTDGAVDEVGLGDYLNRNMLGELRRIDGVGKAQLFSA
QRAMRVWIDPDKMVGLKLTVGDINTAITTQNAQVAAGQIGAAPNPVSQDLTATVLVKGQL
TDIEEFGNIVLRANPDGSTVRLKDVARLEVGAESYNFSSRLNGQPSAAVAIQLSSTGNAV
ATSAAIKAKLDELSRFFPSGVQYDVPYDTSPFVSASIQKVLMTLLEAVGLVFLVMFVFLQ
NFRYTVIPTLVVPIALLGTSAAMLATGFSINVLTMFAMVLAIGILVDDAIVVVENVERIM
AEEGLSPKAATNKAMTQITSAILGITLVLSSVFIPMAFFPGSTGIIYRQFSLTMVVSILF
SAFLALSLTPALCATFLKPIKQGHHDKKGLGGWFNRKFDRLTAGYSRSVTGLVRRAGRVM
VVYLALVVGLGYLFVNLPSGFVPNEDQGYFLVDVQGPPEASANRTVASIKQIEEIFKGES
AVRDVLVIQGFSFSGSGANAAMVFVTLKDWKERGAGNTVDEITERVNMRLFALKDATSFA
LSPPPIEGFGTTNGFSFRLQDRSGLGQQALSSAAAKLMAKAEQSPVLTGLRIEGLPDAAQ
VVLVINREKANTFGVTFADINSTITANLGSSYINDYPNAGRMQRVIVQAQDRDRLQADDL
LKLNVRNAGGGMVPLSSFAIAEWQKGAPQIVGYNGYPTVRIAGEPVPGQSTGAAIAEMER
LASELPEGFGFEWTGQALEEIKSGSQAPMLFGLSILFVFLLLAGLYESWSIPLSVMLVVP
LGAIGCVLAVMMRGMPNDIYFKVGLIAIIGLSAKNAILIVEFAKDYFAQGRSLFDSAVDA
ARVRFRPIVMTSLAFALGVVPLAIATGPSAASQNAIGTGVLGGIILGTILAIFFVPAFFV
FVLKLFRTKRPVEDHEDDKPETGDEVAGKPVA