Protein Info for Rru_A1089 in Rhodospirillum rubrum S1H

Annotation: Holliday junction DNA helicase RuvB (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 PF05496: RuvB_N" amino acids 25 to 183 (159 residues), 264.1 bits, see alignment E=1.3e-82 TIGR00635: Holliday junction DNA helicase RuvB" amino acids 29 to 331 (303 residues), 454.8 bits, see alignment E=6.1e-141 PF06068: TIP49" amino acids 31 to 83 (53 residues), 26.6 bits, see alignment 1.2e-09 PF07728: AAA_5" amino acids 59 to 177 (119 residues), 23.3 bits, see alignment E=2e-08 PF00004: AAA" amino acids 60 to 181 (122 residues), 75.2 bits, see alignment E=2.4e-24 PF17864: AAA_lid_4" amino acids 186 to 259 (74 residues), 99.6 bits, see alignment E=2.3e-32 PF05491: RuvB_C" amino acids 262 to 330 (69 residues), 92.9 bits, see alignment E=3.3e-30

Best Hits

Swiss-Prot: 100% identical to RUVB_RHORT: Holliday junction ATP-dependent DNA helicase RuvB (ruvB) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K03551, holliday junction DNA helicase RuvB (inferred from 100% identity to rru:Rru_A1089)

MetaCyc: 63% identical to Holliday junction branch migration complex subunit RuvB (Escherichia coli K-12 substr. MG1655)
3.1.22.4-RXN [EC: 3.1.21.10]

Predicted SEED Role

"Holliday junction DNA helicase RuvB" in subsystem DNA-replication or RuvABC plus a hypothetical

Isozymes

No predicted isozymes

Use Curated BLAST to search for 3.1.21.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RVF5 at UniProt or InterPro

Protein Sequence (350 amino acids)

>Rru_A1089 Holliday junction DNA helicase RuvB (NCBI) (Rhodospirillum rubrum S1H)
MAGHEEEDERLISGRRREGEVDAALRPQSLADFVGQRQTRENLGVFVQAARARGEAMDHV
LFHGPPGLGKTTLAQIVARELGVGFRGTSGPMIVKAGDLAAILTNLEPRDVLFIDEIHRL
NPAIEEVLYPAMEDFQLDLIIGEGPAARSVRIDLPPFTLVGATTRSGLLTTPLRDRFGIP
LRLDFYETDELVQIVTRGSRLLGMALTDEGAREVARRSRGTPRVAGRLLRRVRDFAAVAG
RSPVDAFIADAALNRLEVDGRGLDAMDRRYLSRMADHYGGGPVGVDTLAAALAEERDTVE
DVIEPYLIQQGFIKRTPRGRMLTAIAWTHLGLTPPAETPPVQPDLWSDAP