Protein Info for Rru_A0994 in Rhodospirillum rubrum S1H

Annotation: Nitrogenase cofactor biosynthesis protein NifB (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 503 TIGR01290: nitrogenase cofactor biosynthesis protein NifB" amino acids 52 to 488 (437 residues), 634.2 bits, see alignment E=5.3e-195 PF04055: Radical_SAM" amino acids 78 to 256 (179 residues), 61.2 bits, see alignment E=1.4e-20 PF02579: Nitro_FeMo-Co" amino acids 393 to 488 (96 residues), 76.2 bits, see alignment E=2e-25

Best Hits

Swiss-Prot: 60% identical to NIFB_SINFN: FeMo cofactor biosynthesis protein NifB (nifB) from Sinorhizobium fredii (strain NBRC 101917 / NGR234)

KEGG orthology group: K02585, nitrogen fixation protein NifB (inferred from 100% identity to rru:Rru_A0994)

Predicted SEED Role

"Nitrogenase FeMo-cofactor synthesis FeS core scaffold and assembly protein NifB" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RVQ0 at UniProt or InterPro

Protein Sequence (503 amino acids)

>Rru_A0994 Nitrogenase cofactor biosynthesis protein NifB (NCBI) (Rhodospirillum rubrum S1H)
MSEQVISLDSISRLGVLSAASRDQVPPPAAAEGCASQGRCGASAGPDDMPAEVWEKVKNH
PCYSEEAHHFFARMHVAVAPACNIQCNYCNRKYDCANESRPGVVSERLTPEQAARKVAAV
ANELPQLSVLGVAGPGDSLFDARKTFETFARVGAMLPDLKFCISTNGLALPDHLDELTRH
AIDHVTITLNCVDPEIGARIYPWIYFKGRRWTGVDGAKILLERQMEGLEGLIARKILVKI
NSVMIPGINDHHLPEVNRVIREKGAFLHNVMPLISAPEHGTHFGLTGQRGPTPAELKDLQ
DRLGGGANLMKHCRQCRADAIGLLGEDRGQEFTMDKIPETVVVDPSRREAYRAFVEEERA
DRRAAAVQTESALARDAAVGTPARLVAITTKGGGRINAHFGKVTEFQIYEVDGAGVRFVG
ARRLPDKYCLGGFDNAPVMPAILDALEGVDAVLTAKIGPGPRGRLEAAGIEVYEDRTLEY
IEPAIGSWYAGRMGAALSERSSA