Protein Info for Rru_A0939 in Rhodospirillum rubrum S1H

Annotation: DNA ligase (NAD+) (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 698 PF01653: DNA_ligase_aden" amino acids 12 to 328 (317 residues), 386.2 bits, see alignment E=3e-119 TIGR00575: DNA ligase, NAD-dependent" amino acids 19 to 692 (674 residues), 786 bits, see alignment E=1.4e-240 PF03120: DNA_ligase_OB" amino acids 331 to 408 (78 residues), 116.5 bits, see alignment E=9.9e-38 PF03119: DNA_ligase_ZBD" amino acids 417 to 444 (28 residues), 40.1 bits, see alignment (E = 6.7e-14) PF12826: HHH_2" amino acids 528 to 599 (72 residues), 50 bits, see alignment E=6.9e-17 PF00533: BRCT" amino acids 623 to 694 (72 residues), 44.5 bits, see alignment E=3.7e-15

Best Hits

Swiss-Prot: 100% identical to DNLJ_RHORT: DNA ligase (ligA) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to rru:Rru_A0939)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RVV5 at UniProt or InterPro

Protein Sequence (698 amino acids)

>Rru_A0939 DNA ligase (NAD+) (NCBI) (Rhodospirillum rubrum S1H)
MIPVADLTEAEAARELERLAAEIARHDRLYHQQDAPEITDGEYDALVRRNNEIEAAFPEL
VRGDSPSAHVGAAPAEGFAKVTHALPMLSLGNVFSDDEAREFDQRIRRFLSLDGETTISY
VAEPKIDGLSFSARYENGVYRRAATRGDGTTGEDITANLATLTDLPQRLTGDGPVPEVLE
VRGEVFMTKADFRALNQRQIDAGAKPFANPRNAAAGSLRQLDPEITRSRSLSLFAYASGE
VKGLEVESHHAFLERLKTWGFPVNPQTRPCQGVEDLIEATHALALQRAALDYDIDGVVYK
VDRVDWQKRLGFVSRAPRWAIARKFPAERAQTTVKAIQIQVGRTGTLTPVAILEPVTVGG
VVVGRATLHNEDEIARKDVRIGDMVTIQRAGDVIPQVVEVLLDQRPADSAPFVFPETCPV
CGSHAVRQEGEVARRCAGGLICSAQAVERLKHFVSRDAFDIEGLGGKHIETFHNEGLITQ
PADIFALEAKDNEPGALQHLRSREGWGPKSAENLFKAITARKTIPLDRFIYALGIRQVGQ
ATARLLAGHYASFAAWRKAMDEAQTEGSEAFADLQNIESIGPSVARDLVAFFGEEHNRAA
LDALIDAGVEVLDFARPQAAGDSPLAGKTVVFTGTLDTMSRGEAKARALALGAKVTGSVS
AKTDYVVVGADAGSKEKKARELGLTVLSEAEFRAMSGG