Protein Info for Rru_A0744 in Rhodospirillum rubrum S1H

Annotation: 2-nitropropane dioxygenase, NPD (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00478: IMPDH
  • amino acids 29 to 223 (195 residues), 26.9 bits, E=3.7e-10
• PF03060: NMO
  • amino acids 30 to 97 (68 residues), 39.9 bits, E=5.2e-14
  • amino acids 102 to 336 (235 residues), 119 bits, E=4.6e-38
• PF05690: ThiG
  • amino acids 141 to 225 (85 residues), 27 bits, E=4.1e-10

Best Hits

KEGG orthology group: K02371, enoyl-[acyl carrier protein] reductase II [EC: 1.3.1.-] (inferred from 100% identity to rru:Rru_A0744)

Predicted SEED Role

"Enoyl-[acyl-carrier-protein] reductase [FMN] (EC 1.3.1.9)" in subsystem Fatty Acid Biosynthesis FASII (EC 1.3.1.9)

MetaCyc Pathways

• superpathway of fatty acids biosynthesis (E. coli) (48/53 steps found)
• superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
• palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
• oleate biosynthesis IV (anaerobic) (13/14 steps found)
• superpathway of unsaturated fatty acids biosynthesis (E. coli) (17/20 steps found)
• superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
• palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (8/9 steps found)
• gondoate biosynthesis (anaerobic) (4/4 steps found)
• (5Z)-dodecenoate biosynthesis I (5/6 steps found)
• stearate biosynthesis II (bacteria and plants) (5/6 steps found)
• cis-vaccenate biosynthesis (4/5 steps found)
• fatty acid elongation -- saturated (4/5 steps found)
• 8-amino-7-oxononanoate biosynthesis I (8/11 steps found)
• (5Z)-dodecenoate biosynthesis II (4/6 steps found)
• anteiso-branched-chain fatty acid biosynthesis (24/34 steps found)
• even iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• odd iso-branched-chain fatty acid biosynthesis (24/34 steps found)
• 8-amino-7-oxononanoate biosynthesis IV (3/5 steps found)
• biotin biosynthesis I (8/15 steps found)
• streptorubin B biosynthesis (20/34 steps found)
• mycolate biosynthesis (26/205 steps found)
• superpathway of mycolate biosynthesis (27/239 steps found)

KEGG Metabolic Maps

• 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
• Biosynthesis of unsaturated fatty acids
• Fatty acid biosynthesis
• Fluorene degradation
• Fluorobenzoate degradation
• Isoflavonoid biosynthesis
• Phenylalanine metabolism
• Propanoate metabolism
• Tryptophan metabolism
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.1.-, 1.3.1.9

Use Curated BLAST to search for 1.3.1.- or 1.3.1.9

Sequence Analysis Tools

See Q2RWE7 at UniProt or InterPro

Protein Sequence (354 amino acids)

>Rru_A0744 2-nitropropane dioxygenase, NPD (NCBI) (Rhodospirillum rubrum S1H)
MKDAAMNGWAEERLDELWARGRAFLGCDTAILGGAMAWVSERHLVSAISGAGGFGVIACG
SMPSDLLSKEIAATRALLEGMGGERPFGVNLITMHPELDRLIDLCGEHRVSHVVLAGGLP
SAASIKRVKDLGARCICFAPALALARKLVRSGADALVIEGAEAGGHIGPVSTSVLAQEIL
PEVPEIPIFVAGGIGRGEAILSYLEMGAAGVQIGTRFVCATECIAHPNFKKAFIRASARD
AVPTIQIDPQFPVIPVRALANKGTARFMEMQRTVIDRFHNGEIDQKSAQLEIEHFWAGAL
RRAVIEGDVDSGSLMAGQSVGMVKREEPVAEILAELRGQAVAALTRRAARQAGG