Protein Info for Rru_A0557 in Rhodospirillum rubrum S1H

Annotation: Methenyltetrahydrofolate cyclohydrolase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 6 to 121 (116 residues), 144.5 bits, E=1.6e-46
• PF02882: THF_DHG_CYH_C
  • amino acids 124 to 291 (168 residues), 218.3 bits, E=3.6e-69

Best Hits

Swiss-Prot: 100% identical to FOLD_RHORT: Bifunctional protein FolD (folD) from Rhodospirillum rubrum (strain ATCC 11170 / ATH 1.1.1 / DSM 467 / LMG 4362 / NCIB 8255 / S1)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 100% identity to rru:Rru_A0557)

MetaCyc: 58% identical to methenylTHF cyclohydrolase/methyleneTHF dehydrogenase subunit (Moorella thermoacetica)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• folate transformations III (E. coli) (7/9 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• L-histidine degradation III (4/6 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (6/9 steps found)
• folate transformations II (plants) (7/11 steps found)
• folate transformations I (8/13 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (3/10 steps found)
• purine nucleobases degradation I (anaerobic) (6/15 steps found)
• purine nucleobases degradation II (anaerobic) (12/24 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (21/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Sequence Analysis Tools

See Q2RWY4 at UniProt or InterPro

Protein Sequence (296 amino acids)

>Rru_A0557 Methenyltetrahydrofolate cyclohydrolase (NCBI) (Rhodospirillum rubrum S1H)
MGAHLIDGKAIAQQLRERIAQEVADLKAHRGLVPGLAVVLVGEDAASQVYVGSKEKMAVA
VGMHSVVCRLAATTSEAEVLAKVEALNADPTIDGILVQLPLPAHIDEDKILLAIDPDKDV
DGFHPVNVGRLSIGQSNGEHGMVPCTPKGCLMLLKGVLGDLTGKTALVIGRSNIVGKPMA
QLLLSANCTVTLAHSRSRDLPDLCRRMDIVVAAVGRPEMVKGDWLKPGAVVIDVGMNRLP
AAPGAEKGKLVGDVDYASAAAVAGAITPVPGGVGPMTIACLLANTLEACKARGAGA