Protein Info for Rru_A0521 in Rhodospirillum rubrum S1H

Annotation: CheA Signal Transduction Histidine Kinases (STHK) (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 942 PF01627: Hpt" amino acids 3 to 95 (93 residues), 59.4 bits, see alignment E=8.5e-20 PF02895: H-kinase_dim" amino acids 246 to 306 (61 residues), 57.3 bits, see alignment 4.2e-19 PF02518: HATPase_c" amino acids 354 to 493 (140 residues), 55 bits, see alignment E=2.7e-18 PF01584: CheW" amino acids 499 to 641 (143 residues), 82.3 bits, see alignment E=6.7e-27 amino acids 671 to 757 (87 residues), 31.6 bits, see alignment E=2.9e-11 PF00072: Response_reg" amino acids 816 to 925 (110 residues), 68.9 bits, see alignment E=1e-22

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 100% identity to rru:Rru_A0521)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RX20 at UniProt or InterPro

Protein Sequence (942 amino acids)

>Rru_A0521 CheA Signal Transduction Histidine Kinases (STHK) (NCBI) (Rhodospirillum rubrum S1H)
MDDLLSEFLTETSESIATLDVELVNLERNPNDKGILSNIFRLVHTIKGTCGFLGLPRLES
LAHAGENVLGKFRDGDLEVTPAAVTVILHTIDRIKEILGHLEANEVEPEGDDKDLKAHLN
AFAEGKQPSVAAMAPAPARAAGSGPAISEGGYPVAAELLAEVSEAVAKGKRAATEAELAA
ELAAELAAAEKAEQAVAAPVPEVIPEVKAATPVVQPAKPPAVTAAHDTNGPNGGGGGEQK
EGSVASQSIRVNVELLENLMTLVSELVLTRNQLLQMVRGSDDSEFVAPLQRLSHITTDLQ
EGVMKTRMQPIGNAWAKLPRIVRDLSIEMHKKIDLQMYGADTELDRQVLEMIKDPLTHMV
RNSGDHGLEFPDERAAAGKPETGVIKLNAYHEGGHIIIEISDDGRGLNLERIRAKALSNG
LATEAELENMTDQQIAQYIFRAGLSTAEKVTAVSGRGVGMDVVKTNIEKIGGTVELKTWP
GKGSRFVIKIPLTLAIVSALIVEASGERFAIPQISVLELVRVTANSETTIEQINTAPVLR
LRDRLMPLVSLSALLRLDDGDDEELGKTADATAGRRDETFIVVSQVGTYTFGIIVDRVFD
TEEIVVKPVAPILRHVSMFSGNTILGDGSVIMILDPNGIASATGEVTMGSASGTTEAAQS
HEFVGEDRTSLLVFRAGGKDLKAVPLALVARLEEIETDKIEHSFGKPVVQYRGQLMPLVG
IHDEFTLAGEGRQPVLVFSDRDRTMGLVVDEIVDIVEDHLKVELRADLRGVVGTAVVNGK
ATDIIDTGYYLTKAFGDWFGTMKSDAFGEQKSAIRVLLVDDSPFFRNLLTPLLSVSGYAV
TAVESAEKALELREKGHSFEAIISDIEMAGMDGFSFAAAIRADGRWGNLPLIALSSHATE
RDLQRGREAGFDDYVAKFDRDSLLEVLGQLVGGQPALVAQEG