Protein Info for Rru_A0505 in Rhodospirillum rubrum S1H

Annotation: Glycogen debranching enzyme GlgX (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 714 TIGR02100: glycogen debranching enzyme GlgX" amino acids 12 to 704 (693 residues), 1041.4 bits, see alignment E=0 PF02922: CBM_48" amino acids 16 to 104 (89 residues), 68.8 bits, see alignment E=4.3e-23 PF00128: Alpha-amylase" amino acids 187 to 359 (173 residues), 48.3 bits, see alignment E=9.6e-17

Best Hits

KEGG orthology group: K02438, glycogen operon protein GlgX [EC: 3.2.1.-] (inferred from 100% identity to rru:Rru_A0505)

Predicted SEED Role

"Glycogen debranching enzyme (EC 3.2.1.-)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 3.2.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.-

Use Curated BLAST to search for 3.2.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RX36 at UniProt or InterPro

Protein Sequence (714 amino acids)

>Rru_A0505 Glycogen debranching enzyme GlgX (NCBI) (Rhodospirillum rubrum S1H)
MSAPSTPLVWPGQPFPLGATWDGSGTNFALFSAHAEKVELCLFDRAGRKETARIVLPEYT
DEIWHGYLPDIRPGQMYGYRVYGPYDPNAGHRFNANKLLIDPYAKSLKGEILWHDALFAY
KLGHPDGDLTFDRRDSARHMPKCEVVDPAFTWGRSMEPHPWHETVIYEMHPRGFTMLHPG
VPEELRGTFDGLAHRRVTDYLTDLGVTAIELLPVHAYVQDRHLIDRGLTNYWGYNSIGFL
APHATYLGHGRTASAFKAFVQKMHDAGLEVILDVVYNHTAEGNHLGPTLSFKGIDNRSYY
YLMGEDLRHYNNFTGTGNALELRHPRVLGMVMDSLRYWATAMGVDGFRFDLATTLARVEG
PFTEHAGFLDAVAQDPVLSHVKLIAEPWDTGLGGYQVGAFPPGWAEWNDQYRNTIRKFWK
GDDGLLPTMAGRFSASSDVFNRRGRRPWASVNFITAHDGFTLADLVSYNGKHNEANGEDN
RDGSDDNNSWNCGAEGPTDDEEINTLRRRQMRNMLATLLLSQGTPMLLAGDEFANSQNGN
NNAYCQDNALSWLDWDGIDEKARSQIAFVTSLLRLRREHVVFHRTRFFTGSVIPGTEVKD
VVWLRPDGEEMTEADWGNSVSQALAIRLSGEAGLTHLDRRGRQQTDDTFLLVLNASHTEV
VFTLPEGGRWETVIDTMDEDGQSSLPKADGGTIRALPGRSLQVLRCRAAHGRSA