Protein Info for Rru_A0493 in Rhodospirillum rubrum S1H
Annotation: Phytoene desaturase (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 53% identical to CRTI_RHOS4: Phytoene desaturase (neurosporene-forming) (crtI) from Rhodobacter sphaeroides (strain ATCC 17023 / 2.4.1 / NCIB 8253 / DSM 158)
KEGG orthology group: K10027, phytoene dehydrogenase [EC: 1.14.99.-] (inferred from 100% identity to rru:Rru_A0493)MetaCyc: 57% identical to phytoene desaturase (lycopene-forming) (Rubrivivax gelatinosus)
RXN-12414 [EC: 1.3.99.31]
Predicted SEED Role
"Phytoene dehydrogenase (EC 1.14.99.-)" in subsystem Carotenoids (EC 1.14.99.-)
MetaCyc Pathways
- neurosporene biosynthesis (2/5 steps found)
- trans-lycopene biosynthesis I (2/6 steps found)
- okenone biosynthesis (2/6 steps found)
- β-carotene biosynthesis (engineered) (2/8 steps found)
KEGG Metabolic Maps
- 1,1,1-Trichloro-2,2-bis(4-chlorophenyl)ethane (DDT) degradation
- Benzoate degradation via hydroxylation
- Biosynthesis of phenylpropanoids
- Biosynthesis of plant hormones
- Biosynthesis of terpenoids and steroids
- Carotenoid biosynthesis - General
- Naphthalene and anthracene degradation
- Nicotinate and nicotinamide metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.14.99.-
Use Curated BLAST to search for 1.14.99.- or 1.3.99.31
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RX47 at UniProt or InterPro
Protein Sequence (507 amino acids)
>Rru_A0493 Phytoene desaturase (NCBI) (Rhodospirillum rubrum S1H) MKLHPAPTVPTQDRRPHAVVIGSGFGGLAAAVRLGARGYRVTVLEKLGGPGGRGGVFKQD GFTFDAGPTIVTLPSLFEELWTLCGRQMSDDVDLRPLTPFYTIRFNDGETFRAQSDPEAM RAEVARLSPGDVEGYERFIAESEDIYKVGFEQMADKPFSSITDMLKVVPQMLKLGALRSV WGHVAKHVADPRLRIALSFHPLFIGGNPLSVTSIYSMIAFLERRWGVHFAMGGTHSLVKG LVGLIEGQGNHLRYNAGVAEILVKNDRACGVRLETGEEIAADIVVSNADSAATYKNLLPA AHRRWWSDRRLAWSRYSMSLFIWHFGTKGTYPDVAHHTILMGPRYHTLIADIFQAKTLAK DFSLYLYRPTATDPSMAPEGCDSFYVLSPVPHLGGGQDWALEAEPYRKAILAELERTVLP GLGAKIVTSKVVTPVDFRDGLSSLNGAAFGLEPAFTQSAWFRPHNKSEEIDRLYLVGAGT HPGAGLPGVLSSAKILDTVVPDASVLV