Protein Info for Rru_A0454 in Rhodospirillum rubrum S1H

Annotation: Leucyl aminopeptidase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 PF02789: Peptidase_M17_N" amino acids 17 to 145 (129 residues), 73 bits, see alignment E=2.2e-24 PF00883: Peptidase_M17" amino acids 183 to 487 (305 residues), 430 bits, see alignment E=5.5e-133

Best Hits

Swiss-Prot: 59% identical to AMPA_RHOPS: Probable cytosol aminopeptidase (pepA) from Rhodopseudomonas palustris (strain BisB5)

KEGG orthology group: K01255, leucyl aminopeptidase [EC: 3.4.11.1] (inferred from 100% identity to rru:Rru_A0454)

Predicted SEED Role

"Cytosol aminopeptidase PepA (EC 3.4.11.1)" (EC 3.4.11.1)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.11.1

Use Curated BLAST to search for 3.4.11.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RX86 at UniProt or InterPro

Protein Sequence (500 amino acids)

>Rru_A0454 Leucyl aminopeptidase (NCBI) (Rhodospirillum rubrum S1H)
MKVSFAKPEIPAQGALVLGVLEGAQLSPLGREVDTQTDGALTRAIAAYPRFKGEAGQSLS
LAAPHGLTASRVLLIGLGKADGLSASGLETFGASVQSTLNHTGDSEGVVVIEAALLGGFD
LGEAAARVALGARLRSYRFDKYHTTQKDDEKPQLATLTVAVEDPAAAEAAYAPLRALAKG
VFLTRDLVSEPANVLYPESFAAQCEDLKKLGIEVEVLGEKEMRKLGMGALLGVAQGSARE
PKLVVLSYHGGKKGDQPVAFIGKGVCFDSGGISIKPAANMEDMKWDMGGAGVVAGLFKAL
AGRKAKVNAVGLMGLVENMPSGTAQRPGDVVTSMSGQTIEVINTDAEGRLVLADVLHYCN
DRFKPRQMIDLATLTGAIIIALGTEHAGVFSNDDALAEGLAKAGKATGEKVWRLPLGDAY
DKQINSDIADMRNSTGREGGSITAAQFLKRFVGETPWAHIDIAGVTWSKKDAGIVPKGGT
GFGVRLLDAYVAETFEEAGK