Protein Info for Rru_A0394 in Rhodospirillum rubrum S1H

Annotation: Amino-acid N-acetyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00120: glutamate N-acetyltransferase/amino-acid acetyltransferase
  • amino acids 18 to 412 (395 residues), 393.8 bits, E=4.2e-122
• PF01960: ArgJ
  • amino acids 25 to 412 (388 residues), 464.6 bits, E=1e-143

Best Hits

Swiss-Prot: 58% identical to ARGJ_RHIME: Arginine biosynthesis bifunctional protein ArgJ (argJ) from Rhizobium meliloti (strain 1021)

KEGG orthology group: K00620, glutamate N-acetyltransferase / amino-acid N-acetyltransferase [EC: 2.3.1.1 2.3.1.35] (inferred from 100% identity to rru:Rru_A0394)

Predicted SEED Role

"Glutamate N-acetyltransferase (EC 2.3.1.35) / N-acetylglutamate synthase (EC 2.3.1.1)" in subsystem Arginine Biosynthesis extended (EC 2.3.1.1, EC 2.3.1.35)

MetaCyc Pathways

• L-arginine biosynthesis II (acetyl cycle) (10/10 steps found)
• superpathway of arginine and polyamine biosynthesis (14/17 steps found)
• L-arginine biosynthesis I (via L-ornithine) (8/9 steps found)
• L-arginine biosynthesis III (via N-acetyl-L-citrulline) (7/9 steps found)
• L-ornithine biosynthesis I (4/5 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.1.1 or 2.3.1.35

Sequence Analysis Tools

See Q2RXE6 at UniProt or InterPro

Protein Sequence (412 amino acids)

>Rru_A0394 Amino-acid N-acetyltransferase (NCBI) (Rhodospirillum rubrum S1H)
MTHAVSPLAPAAFPALPAIAGVDLATHNTGLRYQGRPDLLVVRLAAGSTGAGVFTRSKTR
SAPVDWCRRALAAGGGAARGLVVNSGNANAFTGSRGIASVERTIASAAMALSCPPEEVFI
ASTGTIGVPLDDAKITTLIDAIAPSLGTASWFDAATAISTTDTYPKGATRTATIDGVSVT
LSGIAKGSGMIAPDMATMLAYVFTDAALPAGVLQDLLTQGVERSFNAITVDGDTSTSDSL
MLFATGKAGNAPVQTALDRRLSGFRKALFALLTDLAHQVVRDGEGATKFVTVTVTGAKSP
RAAKRIGLAIANSPLVKTAIAGEDANWGRIVMAVGKSGEEADRDRLEIRIGGHLIAAEGQ
AVADYDEGPVATHMKGTDVDIAVDLGLGRGKATVWTCDLTHGYIDINADYRT