Protein Info for Rru_A0385 in Rhodospirillum rubrum S1H

Annotation: Gamma-glutamyltransferase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 signal peptide" amino acids 1 to 24 (24 residues), see Phobius details TIGR00066: gamma-glutamyltransferase" amino acids 43 to 567 (525 residues), 623.5 bits, see alignment E=1.3e-191 PF01019: G_glu_transpept" amino acids 52 to 573 (522 residues), 617.3 bits, see alignment E=1.3e-189

Best Hits

Swiss-Prot: 59% identical to GGT_ECOLI: Glutathione hydrolase proenzyme (ggt) from Escherichia coli (strain K12)

KEGG orthology group: K00681, gamma-glutamyltranspeptidase [EC: 2.3.2.2] (inferred from 100% identity to rru:Rru_A0385)

MetaCyc: 59% identical to glutathione hydrolase proenzyme (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Gamma-glutamyltranspeptidase (EC 2.3.2.2)" in subsystem Glutathione: Biosynthesis and gamma-glutamyl cycle or Utilization of glutathione as a sulphur source (EC 2.3.2.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.2.2

Use Curated BLAST to search for 2.3.2.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RXF5 at UniProt or InterPro

Protein Sequence (576 amino acids)

>Rru_A0385 Gamma-glutamyltransferase (NCBI) (Rhodospirillum rubrum S1H)
MGVLKVVVTTGLAFAALTLPSMAQEKAIFDPMDRIHPVWAEHGMVAAQERLAAEIGRDIL
AKGGNAVDAGVAVAFALAVTLPQAGNIGGGGFMIVHDGPTGKTVAIDYREKAPAGASRDM
FLDAQGNADSGKSLYSGAASGVPGTVAGMKAALDAFGSMDWAEVIAPAIRLAEGGIVVTP
KLADSLKDARKQLEAFPSSARIFLKEGGADYRPGDRLVQADLAATLKTIAAEGPDGFYKG
AVAEKISAAVQAAGGLITPQDMADYTVVMREPVRGGYRGYEIVSMPPPSSGGVHLVQILN
ALEGYPIGALGQNTGQTLHLMAETMKLAYADRAHYLGDPDFTAVPVAALTSKDYAAAMRE
TISADFARPSATIGPGDLTPYESDQTTHFSIIDKNGSAVANTYTLNLSYGSGLVAEGTGV
LMNNEMDDFSAKPGVPNAFGLVGGDANAVQAGKRPLSSMTPTIVLKDGKVWLVTGSPGGA
RIITTVLQVIMNMIDHKMNVAEASTAPRAHHQWLPDELRIEEGISLDTIRILQAKGHTIA
LKPAMGSTQSIMRDGASGAVYGASDPRDPDAATVGY