Protein Info for Rru_A0377 in Rhodospirillum rubrum S1H

Annotation: Signal Transduction Histidine Kinase (STHK), LytS (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 576 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details amino acids 46 to 64 (19 residues), see Phobius details amino acids 76 to 96 (21 residues), see Phobius details amino acids 105 to 130 (26 residues), see Phobius details amino acids 142 to 164 (23 residues), see Phobius details amino acids 176 to 197 (22 residues), see Phobius details PF07694: 5TM-5TMR_LYT" amino acids 30 to 198 (169 residues), 200.4 bits, see alignment E=2.5e-63 PF06580: His_kinase" amino acids 373 to 450 (78 residues), 86.7 bits, see alignment E=1.6e-28

Best Hits

Swiss-Prot: 65% identical to BTSS_ECOLI: Sensor histidine kinase BtsS (btsS) from Escherichia coli (strain K12)

KEGG orthology group: K02478, two-component system, LytT family, sensor kinase [EC: 2.7.13.3] (inferred from 100% identity to rru:Rru_A0377)

MetaCyc: 65% identical to high-affinity pyruvate receptor (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Autolysin sensor kinase (EC 2.7.3.-)" (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RXG3 at UniProt or InterPro

Protein Sequence (576 amino acids)

>Rru_A0377 Signal Transduction Histidine Kinase (STHK), LytS (NCBI) (Rhodospirillum rubrum S1H)
MTIGPLALVISLSQQMCVYLVVAYLLSKTPLFIPLVHVTIRLPHKAACYAIFSLFCIMGT
YFGLRIDDSIANTRAIGAVLGGMLGGPSVGLAVGLTGGLHRYSMGGFTALACAISTTAEG
LIGGLVHRYLVSRGQTGKLFQPLVVGLVTLFAEVCQMLIIILVARPAVNAVHLVETIALP
MLVANSVGAAMFMTILLDRRTMFETQSSVFSAKALKIAARAEGALRSGFNRENSMRVARI
IYEETGVGAVAITDRDKILAFIGIGDDHHKPGMAVTSLQTLESIRENRVMYADGNEVAYQ
CSISPTCPLGAALVIPLVDEGEQVIGTIKLYEPKTRLFSTINRTLGEGVARLLSTQILAG
RYEQQRQLLAQSEIKLLHAQVNPHFLFNALNTLSAVIRLDPDHARHLVQSLSTFFRKNLK
RTSEQVTLNDEVEQVSAYLEIELARFGDRLSVDFNVPADLGTATLPTFSLQPLVENAIKH
GTAQLLGDGRIGVSAERQGGDLVVRVEDNAGLYERRPGGDGLGMNLVDRRIRARYGQGYG
LSVECERDVVTRVILRVPFDPSPAGVASPAFEKVGP