Protein Info for Rru_A0322 in Rhodospirillum rubrum S1H

Annotation: Peptidase M52, hydrogen uptake protein (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 154 TIGR00072: hydrogenase maturation protease" amino acids 16 to 152 (137 residues), 111 bits, see alignment E=2.5e-36 PF01750: HycI" amino acids 37 to 145 (109 residues), 51.3 bits, see alignment E=5.7e-18

Best Hits

Swiss-Prot: 35% identical to HYCI_ECO57: Hydrogenase 3 maturation protease (hycI) from Escherichia coli O157:H7

KEGG orthology group: K08315, hydrogenase 3 maturation protease [EC: 3.4.-.-] (inferred from 100% identity to rru:Rru_A0322)

MetaCyc: 35% identical to hydrogenase 3 maturation protease (Escherichia coli K-12 substr. MG1655)
RXN-22655 [EC: 3.4.23.51]

Predicted SEED Role

"Coenzyme F420 hydrogenase maturation protease (EC 3.4.24.-)" in subsystem Coenzyme F420 hydrogenase or Hydrogenases (EC 3.4.24.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.4.-.-, 3.4.24.-

Use Curated BLAST to search for 3.4.-.- or 3.4.23.51 or 3.4.24.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RXL8 at UniProt or InterPro

Protein Sequence (154 amino acids)

>Rru_A0322 Peptidase M52, hydrogen uptake protein (NCBI) (Rhodospirillum rubrum S1H)
MTIFDDLGGRLKGRVLVVGAGNPLRGDDGAGPRLVEMLTGKVNAALLDVGEIPEAYTRRI
LETRADTILFVDAADFGAQPGDFALLEEETIAAGHAGTHQLPLTLFFQYLRESGGGEIVV
LGIQPQSISLGTPMSAAVSRTLASLSDLLKELLK