Protein Info for Rru_A0316 in Rhodospirillum rubrum S1H

Annotation: NADH-ubiquinone oxidoreductase (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 561 transmembrane" amino acids 280 to 296 (17 residues), see Phobius details PF00329: Complex1_30kDa" amino acids 34 to 150 (117 residues), 91.5 bits, see alignment E=9.8e-30 PF00374: NiFeSe_Hases" amino acids 213 to 282 (70 residues), 49.7 bits, see alignment E=4.4e-17 PF00346: Complex1_49kDa" amino acids 290 to 454 (165 residues), 147.4 bits, see alignment E=7e-47 amino acids 463 to 532 (70 residues), 59.4 bits, see alignment E=4.8e-20

Best Hits

KEGG orthology group: K00329, NADH dehydrogenase [EC: 1.6.5.3] (inferred from 100% identity to rru:Rru_A0316)

Predicted SEED Role

"Formate hydrogenlyase subunit 5" in subsystem Formate hydrogenase

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RXM4 at UniProt or InterPro

Protein Sequence (561 amino acids)

>Rru_A0316 NADH-ubiquinone oxidoreductase (NCBI) (Rhodospirillum rubrum S1H)
MTTPQTILDDINARFPGCVTRGLAPQPDRLYLRTTTEHIRTLAAYVFNDLKGRLVTGVCT
DMGPVTGEYDIIYAFSLDAEGILLTLTEPTDPKDPKVPTLTDLIPGADWHERENYDMLGV
VAEGHPNPRRLLLSEDWPEDLFPMRKDFPHDFKPPKAEKITTPLRDPLGNESKATIISVG
PFFPTLDEPAYFRLFCEGEEIIGSDYRGFFSHRGIEKLSDTVLDYNQVPFMAERVCGICG
FVHSACYCMAVEDAAGIEIPPRAKYIRSIMMELERLHSHLLWLGLAGHYLGFDTVLMQSW
RIREPIMWLVEEITGNRKTYGMNLVGGVRRDLDSAICDKIMAAVTKIGIECEELISAVAG
DESLKMRMVKVGVLSHEDARGICVVGPTARASGVHIDARADYPYAAFPDLDFKPSFHEGG
DIWARTLVRVDEVRSSVDLIKQMITKLPEGEIMAGFGPIPAWREGYGIAEAPRGECVHYV
QTGKDNRPYRWRVRAATYPQLQAVPLMLKGMSIGDFPIIIGSIDPCFSCTERVLTVDTKS
KAIRTYTEKDLLALTRRAGGR