Protein Info for Rru_A0308 in Rhodospirillum rubrum S1H

Annotation: Hydrogenase maturation protein HypF (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 768 PF00708: Acylphosphatase" amino acids 24 to 105 (82 residues), 62.9 bits, see alignment E=5.4e-21 TIGR00143: carbamoyltransferase HypF" amino acids 26 to 756 (731 residues), 651 bits, see alignment E=1.1e-199 PF07503: zf-HYPF" amino acids 125 to 156 (32 residues), 47.9 bits, see alignment (E = 1.8e-16) amino acids 174 to 206 (33 residues), 55.3 bits, see alignment (E = 9e-19) PF01300: Sua5_yciO_yrdC" amino acids 215 to 404 (190 residues), 128.5 bits, see alignment E=4e-41 PF17788: HypF_C" amino acids 419 to 513 (95 residues), 110.4 bits, see alignment E=1.2e-35

Best Hits

Swiss-Prot: 51% identical to HYPF_AZOCH: Carbamoyltransferase HypF (hypF) from Azotobacter chroococcum mcd 1

KEGG orthology group: K04656, hydrogenase maturation protein HypF (inferred from 100% identity to rru:Rru_A0308)

Predicted SEED Role

"[NiFe] hydrogenase metallocenter assembly protein HypF" in subsystem NiFe hydrogenase maturation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RXN2 at UniProt or InterPro

Protein Sequence (768 amino acids)

>Rru_A0308 Hydrogenase maturation protein HypF (NCBI) (Rhodospirillum rubrum S1H)
MGPITMGLSPDLPGSSPAPATRLRLSVRGLVQGVGFRPTVYRLATGLGLRGFVLNTGAGV
VIEVEGARAGEFAEILRHGAPPRAHIEALETHRLAASGRDEGFRIIDSDTAAPGTTRIGP
DTAPCPSCLSELFDPADRRYRHPFIACCDCGPRHTIAGALPYDRARTAMGGFALCAACAA
DYADPASRRFHAELTACPDCGPRLTLDPADILREIRAGAIVALKGLGGFQLICDASQPAP
VERLRRGKRRDAKPFALLVASLTDARALCDVRSAEAEALTSPERPIVLLHRRADARPPLA
AAIAPGLDTLGVMLPVTPLQYLIFHEAAGRPAGTAWLDQPTPLVLVATSANRAGAPLILE
GAEVAAELGADVDVLADHDRPILARADDGVVRVLGDQARSVRRGRGQTPEPIVLGRALPP
VLALGAQWKNSVCVTRGDRAFLSQHIGDLDSRATLAFQRAAIDQLMRFVDTRPVVVAHDL
HPDFASSRLAEAMGLPTIAIQHHHAHLAAVAAEHHIDGPLVGLALDGFGLGDDGGAWGGE
LLRLDGDGFVRLGHLAPLALPGGDAAARQPWRMAASALARLGRGDEIVRRFAHRPEAGQI
AALLARPDTLGQTTSCGRWFDAAAGLLGLCDEVRYEGEAAMRLEALAASPRIAAEGWRIS
QGVLDLLPLLDRLSRLDAGAGAGLFHGTLVAALVDWALPAIHACPGARVALAGGCLINRS
LAEGLITTFAARGITALLPRQSPANDGGLALGQAYLAGLRWLRLAKEA