Protein Info for Rru_A0156 in Rhodospirillum rubrum S1H

Annotation: Secretion protein HlyD (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 transmembrane" amino acids 30 to 51 (22 residues), see Phobius details PF16576: HlyD_D23" amino acids 68 to 311 (244 residues), 73.6 bits, see alignment E=2.1e-24 PF13533: Biotin_lipoyl_2" amino acids 71 to 115 (45 residues), 42.5 bits, see alignment 6.5e-15 PF13437: HlyD_3" amino acids 236 to 315 (80 residues), 51.5 bits, see alignment E=2.3e-17

Best Hits

KEGG orthology group: K03543, multidrug resistance protein A (inferred from 100% identity to rru:Rru_A0156)

Predicted SEED Role

"Membrane fusion component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RY34 at UniProt or InterPro

Protein Sequence (390 amino acids)

>Rru_A0156 Secretion protein HlyD (NCBI) (Rhodospirillum rubrum S1H)
MSTAPSGPRSTVSAEPTLPPASPRSWGRRLLLGVLVVGAVAAAGVFGWRWWTEGRFLETT
DNAYIQGDITAIAPKVSGYVDEVAVGDNEVVSAGQLLVRLDSADYRVQVDQARANAEGKA
AELASIIANIDLQQTAIAQARGAMDAAQARFDRAAKDLARYQDLVGRGATSRQSLDSARE
QATSGAAELRSAAAALDGAQKRLAVLSADRDAAAAEVRSAQAQRALAEINLANTEIRAPI
AGVVGNRGVRAGQYVAPGSALMAIVPLEGLWIAANFKETQLGAMAPGQTVKVEIDAYSGR
HGQGRVVSFAPASGAQFSLLPPENATGNFTKVVQRVPVRIEMLADDPLSALMRPGLSVEV
SVDTRGGRDVALGLVGTALAGSAGSSPTHR