Protein Info for Rru_A0035 in Rhodospirillum rubrum S1H

Annotation: Cytochrome c-type biogenesis protein CcmF (NCBI)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 671 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details amino acids 44 to 64 (21 residues), see Phobius details amino acids 96 to 115 (20 residues), see Phobius details amino acids 126 to 147 (22 residues), see Phobius details amino acids 178 to 197 (20 residues), see Phobius details amino acids 209 to 231 (23 residues), see Phobius details amino acids 251 to 267 (17 residues), see Phobius details amino acids 276 to 299 (24 residues), see Phobius details amino acids 314 to 333 (20 residues), see Phobius details amino acids 354 to 376 (23 residues), see Phobius details amino acids 396 to 415 (20 residues), see Phobius details amino acids 427 to 446 (20 residues), see Phobius details amino acids 452 to 473 (22 residues), see Phobius details amino acids 497 to 519 (23 residues), see Phobius details amino acids 624 to 641 (18 residues), see Phobius details TIGR00353: cytochrome c-type biogenesis protein CcmF" amino acids 53 to 647 (595 residues), 670.8 bits, see alignment E=1.1e-205 PF01578: Cytochrom_C_asm" amino acids 89 to 297 (209 residues), 176.5 bits, see alignment E=5.8e-56 PF16327: CcmF_C" amino acids 317 to 644 (328 residues), 387.9 bits, see alignment E=4e-120

Best Hits

Swiss-Prot: 56% identical to CCMF_BRADU: Cytochrome c-type biogenesis protein CycK (cycK) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02198, cytochrome c-type biogenesis protein CcmF (inferred from 100% identity to rru:Rru_A0035)

Predicted SEED Role

"Cytochrome c heme lyase subunit CcmF" in subsystem Biogenesis of c-type cytochromes or Copper homeostasis

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q2RYF5 at UniProt or InterPro

Protein Sequence (671 amino acids)

>Rru_A0035 Cytochrome c-type biogenesis protein CcmF (NCBI) (Rhodospirillum rubrum S1H)
MIAELGHFCLVLALFVALVQAVVPMVGAQRGNLAWMDLARSGSVALFGLIVLAFAALTYC
YVVSDFSVMNVFENSHSDKPMLYKVSGVWGNHEGSLLLWILILSGFSLAVSLVGGNLPPA
LRARTLSVQAMITCGFLLFILFTSNPFARVPGLEVPLNGRGLNPLLQDPGLAFHPPMLYA
GYVGFSMAFSFAIAALIEGKVDAAWARWVRPWTLVAWVFLTFGIALGSWWAYYELGWGGW
WFWDPVENASFIPWLSGTALLHSAVVVEKRDALKSWTILLAIVTFSLSLLGTFLVRSGVI
TSVHAFATDPTRGLFILGLLLVAIGGSFTLYAVRAPALKGGGLFAPISREGGLLINNLLM
STASATVLLGTLYPLIADALDLGKVTVGAPFFNRVFLPLMVPLLVLMAIGPLLSWKRADL
RGALGRLKGALIVAALVFAGTFLATGGGLAQVGAAAGMTLAAWVLVGTLVELADRIRFLR
IPLADSLRRLVGLPRSTWGMVLAHGGMGLVVIGVVGNTAWKAESIQTLRPGESRELAGHT
FTLQGAAPIQGPNYEALRGDILLSREGETLARLAPEKRLYTTPPMPTTEAGIHSNGLSDY
YAVIGDPTDNGGFVTRFYYEPAVPFLWYGALFMGLGGLVSLSDRRHRVGAPTARRVSPAK
TASPPAPAPAE