Protein Info for Rru_A0019 in Rhodospirillum rubrum S1H
Annotation: Glutamate synthase (ferredoxin) (NCBI)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 72% identical to GLTB_AZOBR: Glutamate synthase [NADPH] large chain (gltB) from Azospirillum brasilense
KEGG orthology group: K00265, glutamate synthase (NADPH/NADH) large chain [EC: 1.4.1.13 1.4.1.14] (inferred from 70% identity to acr:Acry_1666)MetaCyc: 72% identical to glutamate synthase alpha subunit (Azospirillum brasilense)
Glutamate synthase (NADPH). [EC: 1.4.1.13]
Predicted SEED Role
"Glutamate synthase [NADPH] large chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)
MetaCyc Pathways
- L-asparagine biosynthesis III (tRNA-dependent) (4/4 steps found)
- glutaminyl-tRNAgln biosynthesis via transamidation (4/4 steps found)
- ammonia assimilation cycle III (3/3 steps found)
- L-glutamate biosynthesis I (2/2 steps found)
- ammonia assimilation cycle I (2/2 steps found)
- L-glutamate biosynthesis IV (1/1 steps found)
- L-glutamine degradation I (1/1 steps found)
- L-glutamine degradation II (1/1 steps found)
- L-citrulline biosynthesis (6/8 steps found)
- superpathway of ammonia assimilation (plants) (2/3 steps found)
- superpathway of L-citrulline metabolism (8/12 steps found)
- L-glutamate and L-glutamine biosynthesis (4/7 steps found)
KEGG Metabolic Maps
- Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
- Glutamate metabolism
- Nitrogen metabolism
Isozymes
Compare fitness of predicted isozymes for: 1.4.1.13, 1.4.1.14
Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q2RYH1 at UniProt or InterPro
Protein Sequence (1524 amino acids)
>Rru_A0019 Glutamate synthase (ferredoxin) (NCBI) (Rhodospirillum rubrum S1H) MTQSSERLSASAPESGEAFVARWQRDAARLEAAHAYDPAHEHSSCGVGLVASINGKPRRS VVEAGIQALKVLFHRGAVDADGKTGDGAGIHVEIPQDFFKEHIRRTTHEPRAGRVAVGMV FLPKTDLNAQEVCRTIVESEILSFGYSIYGWRQVPVDTSVIGEKANATRPEIEQIMIANT RGGDDEQFERDLYVIRRRIEKHALASNVGELYICSLSCRSIIYKGMFLAEQLTSFYPDLL DERFISSFAIYHQRYSTNTFPTWRLAQPFRMLAHNGEINTLTGNINWMKAHETRMDSPFF AEVIEDLKPVVQPGGSDSAALDGVFELLCRAGRPAPMVKAMVIPESLTQNALMPDHHKAL IGYCNCVMEPWDGPAAIAATDGRWVVAGLDRNGLRPMRFTITTDGLLIVGSETGMVRVPE SDILRKGRLGPGGMVAVDLREGRLYEDGEIKDVLAAREPYAEWVGKITELEGQIGTDVVE PATLSTEDLCRRQLACGQSMEDLELILHPMVADAKEALGSMGDDAPLAVLSSHYRALHHF FRQNFSQVTNPPIDALRETRVMSLKTRLGNLGNILDEDASQCDLLQLESPVLTTAEFEAM RRSMGETVAEVDCTFSPDGGPTGLRDAIVRVQQEAEEAVRGGCTHVVLTDTALSATRVAI PMILAAGAVHTHLIREQLRTFTSLNVRCAEVLDVHYFAVLIGVGATTVNPWLAQESIADR LRRGLFDEGVTLEAAMKRFKKAVNEGLLKIMSKMGISVISSYRGGCNFEAIGLSRSLVAE YFPSLTSRISGIGLAGIQKKVLEQHAKAFGAAAVVLPVGGFYRVRKQGGEAHHFDGDLIH TLQQAVATDSYQTFKKYTAGLRKLPPITIRDLLDFKPGTTGVSIEEVESITSIRRRFVTP AMSLGALSPEAHGTLNIAMNRIGAKSDSGEGGEVRERYKPAANGDNANSAIKQVASGRFG VTAEYLNQCREIEIKVAQGAKPGEGGQLPGFKVTEMIAKLRHSTPGVMLISPPPHHDIYS IEDLAQLIYDLKQINPRAKVCVKLVSRSGIGTIAAGVAKANADVILISGHSGGTGASPQT SIKHAGLPWEIGLAEVHQVLTLNRLRHRVTLRTDGGIKCGRDVVIAAMLGAEEYGIGTTS LVAMGCIMVRQCHSNTCPVGVCTQDDDLRAKFTGTAEKVVNLFSFMAEEVKEILASLGMR SLDEVVGRTDLLQQVSRGADHLDDLDLNPILVQPDTGSGPNICTMEGRNEVPETLDAQMI ADAKSALDDGEKMQLTYTVRNTHRAIGTKLSHRIVIKYGMTGLEPGHITVRLRGSCGQSL GAFAVQGLKLEVFGDSNDYVGKGLSGGTIVVRPAVSSPLQTNENTIIGNTVLYGATSGKL FAAGQAGERFAVRNSGAQVVVEGCGSNGCEYMTNGTVVILGQVGANFGAGMTGGMAFVYD PQNTFPLRVNPDSVIWQRIEVPHYAGVLRDLIAEHVSETQSKYAEHLLADWGREIGNFWQ IVPKEMVNRLAIPARVAEAEKVSA