Protein Info for Xcc-8004.6.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: DNA gyrase subunit B (EC 5.99.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 814 TIGR01059: DNA gyrase, B subunit" amino acids 15 to 814 (800 residues), 978.3 bits, see alignment E=9.1e-299 PF02518: HATPase_c" amino acids 42 to 182 (141 residues), 61.4 bits, see alignment E=3.2e-20 PF00204: DNA_gyraseB" amino acids 231 to 400 (170 residues), 196.2 bits, see alignment E=9.2e-62 PF01751: Toprim" amino acids 429 to 538 (110 residues), 64.5 bits, see alignment E=2.4e-21 PF21249: GyrB_hook" amino acids 543 to 570 (28 residues), 28.8 bits, see alignment (E = 2.2e-10) PF18053: GyrB_insert" amino acids 574 to 740 (167 residues), 159.1 bits, see alignment E=2.8e-50 PF00986: DNA_gyraseB_C" amino acids 743 to 803 (61 residues), 104.6 bits, see alignment 7.1e-34

Best Hits

KEGG orthology group: K02470, DNA gyrase subunit B [EC: 5.99.1.3] (inferred from 100% identity to xcb:XC_0004)

Predicted SEED Role

"DNA gyrase subunit B (EC 5.99.1.3)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.3)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.3

Use Curated BLAST to search for 5.99.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H2X298 at UniProt or InterPro

Protein Sequence (814 amino acids)

>Xcc-8004.6.1 DNA gyrase subunit B (EC 5.99.1.3) (Xanthomonas campestris pv. campestris strain 8004)
MTDEQTTPPTPNGTYDSSKITVLRGLEAVRKRPGMYIGDVHDGTGLHHMVFEVVDNSVDE
ALAGHADDIVVKIHVDGSVAVSDNGRGVPVDIHKEEGVSAAEVILTVLHAGGKFDDNSYK
VSGGLHGVGVSVVNALSEHLWLDIWRDGFHYQQEYALGEPQYPLKQLEASTKRGTTLRFK
PAVEIFSDVEFHYDILARRLRELSFLNSGVKIALIDERGEGRRDDFHYEGGIRSFVEHLA
QLKTPLHPNVISVTGEHNGIVVDVALQWTDAYQETMYCFTNNIPQKDGGTHLAGFRGALT
RVLSNYIEQNGIAKQAKITLTGDDMREGMIAVLSVKVPDPSFSSQTKEKLVSSDVRPAVE
NAFGARLQEFLQENPNEAKAITGKIVDAARAREAARKARDLTRRKGALDIAGLPGKLADC
QEKDPALSELFIVEGDSAGGSAKQGRNRKNQAVLPLRGKILNVERARFDRMLASDQVGTL
ITALGTGIGRDEYNPDKLRYHRIILMTDADVDGSHIRTLLLTFFYRQMPELIERGYIYIG
LPPLYKLKQGKSELYLKDDAALNAYLASSAVEGAALIPASDEPPITGEALEKLLLLFAGA
KEAIARNAHRYDPALLTALIDLPPLDVVQLQAEGDVHPTLDALQAVLNRGTLGTARYHLR
FDPATDSAAASLVSVRKHMGEEFTQVLPMGAFESGELRPLREVALALHGLVREGAQILRG
NKSHPITSFAQAQAWLLEEAKRGRQVQRFKGLGEMNAEQLWETTVNPDTRRLLQVRIEDA
VAADQIFSTLMGDVVEPRRDFIEDNALKVSNLDI