Protein Info for Xcc-8004.5407.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: GTPase and tRNA-U34 5-formylation enzyme TrmE

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 446 signal peptide" amino acids 1 to 20 (20 residues), see Phobius details PF10396: TrmE_N" amino acids 7 to 120 (114 residues), 134.2 bits, see alignment E=5.4e-43 TIGR00450: tRNA modification GTPase TrmE" amino acids 12 to 446 (435 residues), 322.9 bits, see alignment E=4e-100 PF12631: MnmE_helical" amino acids 123 to 443 (321 residues), 170.6 bits, see alignment E=1e-53 PF01926: MMR_HSR1" amino acids 219 to 314 (96 residues), 83.7 bits, see alignment E=2e-27 PF02421: FeoB_N" amino acids 219 to 307 (89 residues), 31 bits, see alignment E=3.4e-11 TIGR00231: small GTP-binding protein domain" amino acids 220 to 334 (115 residues), 75.7 bits, see alignment E=3.7e-25

Best Hits

Swiss-Prot: 100% identical to MNME_XANC8: tRNA modification GTPase MnmE (mnmE) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K03650, tRNA modification GTPase (inferred from 100% identity to xca:xccb100_4464)

Predicted SEED Role

"GTPase and tRNA-U34 5-formylation enzyme TrmE" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4UNL0 at UniProt or InterPro

Protein Sequence (446 amino acids)

>Xcc-8004.5407.1 GTPase and tRNA-U34 5-formylation enzyme TrmE (Xanthomonas campestris pv. campestris strain 8004)
MNALPSTIVAIATAAGTGGIGIVRLSGPQSVQIAAALGIAGLQSRHARYARFRDAQGEVI
DDGIAVWFPAPHSFTGEEVVELQGHGSPVLLRQLVARCIALGARQARAGEFSERAFLNGK
LDLAQAEAIADLIAAGDLRAARAARRSLDGVFSRRVDAVSESLTRLRIHVEAAIDFADEP
LDTLGGAQVREELTRTRALLAQLLRDAERGRKLRDGLHAVLIGPPNAGKSSLLNALAGSD
RAIVTDVAGTTRDTLHEAIQLDGFELTLVDTAGLREGGDAIEREGMRRARAELQRADLAL
IVLDARDPQAARDALGDAIDAVPRRLWIHNKCDLLAVAGPMDADAIAVSAVTGQGLEHLH
TRLRELALGDGIESVDGEFSARTRHVDALHRAEQHADAADLELRYEQLELAAEELRLAHE
ALGEITGKLSADDLLGKIFSSFCIGK