Protein Info for Xcc-8004.538.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 744 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 132 to 740 (609 residues), 866.3 bits, see alignment E=6.4e-265 PF02922: CBM_48" amino acids 149 to 223 (75 residues), 79.4 bits, see alignment E=3e-26 PF00128: Alpha-amylase" amino acids 291 to 365 (75 residues), 29 bits, see alignment E=1.2e-10 PF02806: Alpha-amylase_C" amino acids 644 to 742 (99 residues), 93.4 bits, see alignment E=1.3e-30

Best Hits

Swiss-Prot: 100% identical to GLGB2_XANC8: 1,4-alpha-glucan branching enzyme GlgB 2 (glgB2) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 99% identity to xca:xccb100_0441)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4UZL7 at UniProt or InterPro

Protein Sequence (744 amino acids)

>Xcc-8004.538.1 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) (Xanthomonas campestris pv. campestris strain 8004)
MSERLGVHGRDAEQEMAEGGGGSAVALQDLQAIAAGLPGDAFAVLGPHVQADGRLRVRVL
APGAEALGLIDGRGKLLARMQASPIDGVFEGELPADAAYRLRIVWPDVVQEIEDPYAFAP
QIDESALLQIGAGDGQALRANLGARHVQVGELPAVRFAVWAPHAQRVAVVGDFNGWEPRR
HPMRQRSGGIWELVLPRVETGARYKYAIITADGRVLLKADPVARQSELPPATASVVASAD
AFAWTDAEWMARRSAAAEPAPLSIYEVHAASWRRDGHDQPLDWVSLAAQLIPYVQELGFT
HIELLPITEHPFGGSWGYQPLGLYAPTARHGSPDGFAQFVDACHRAGIGVILDWVSAHFP
DDAHGLSQFDGSATYEHADPREGMHRDWNTLIYNYGRPEVTAYLLGSAMEWIAHYHLDGL
RVDAVASMLYRDYGRAEGEWVPNAHGGRENLEAVAFLRQLTGEIASQFPGVLTIAEESTA
WPGVTAPISEGGLGFTHKWNMGWMHDTLHYMQRDPAARAQHHSQLTFGLVYAFSERFVLP
LSHDEVVHGTGGLLGQMPGDDWRRFANLRAYLALMWAHPGDKLLFMGAEFGQWADWNHDR
SLDWHLLDHAPHRGMQQLVRDLNRALRRVPALYRGNHRADGFEWSVADDARNSVLAFIRH
DPDGGAPLLAVSNLTPQPHHDYRVGVPRAGGWREILNTDSAHYGGSNLGNGGRLLTEPTG
MHGHAQSLRLTLPPLATIYLQAEK