Protein Info for Xcc-8004.5114.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: ATP-dependent DNA helicase UvrD/PcrA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 723 TIGR01075: DNA helicase II" amino acids 1 to 720 (720 residues), 1209.2 bits, see alignment E=0 PF00580: UvrD-helicase" amino acids 5 to 268 (264 residues), 277 bits, see alignment E=6.4e-86 PF13245: AAA_19" amino acids 9 to 251 (243 residues), 78.9 bits, see alignment E=1.2e-25 PF13361: UvrD_C" amino acids 273 to 616 (344 residues), 270 bits, see alignment E=1.2e-83 PF13538: UvrD_C_2" amino acids 556 to 613 (58 residues), 34.2 bits, see alignment 5.5e-12 PF21196: PcrA_UvrD_tudor" amino acids 676 to 718 (43 residues), 30.2 bits, see alignment 1e-10

Best Hits

Swiss-Prot: 61% identical to UVRD_ECOLI: DNA helicase II (uvrD) from Escherichia coli (strain K12)

KEGG orthology group: K03657, DNA helicase II / ATP-dependent DNA helicase PcrA [EC: 3.6.4.12] (inferred from 100% identity to xca:xccb100_4219)

MetaCyc: 61% identical to DNA helicase II (Escherichia coli K-12 substr. MG1655)
RXN-11135 [EC: 5.6.2.4]

Predicted SEED Role

"ATP-dependent DNA helicase UvrD/PcrA" in subsystem DNA repair, bacterial UvrD and related helicases

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.12

Use Curated BLAST to search for 3.6.4.12 or 5.6.2.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H2XCH6 at UniProt or InterPro

Protein Sequence (723 amino acids)

>Xcc-8004.5114.1 ATP-dependent DNA helicase UvrD/PcrA (Xanthomonas campestris pv. campestris strain 8004)
LLDHLNPAQREAVSAPPGHYLVLAGAGSGKTRVLIHRIAWLNEVQGVPNHGIFAVTFTNK
AAGEMRHRTDLQLRNGSRGMWIGTFHGLAHRLLRLHWQDARLPEGFQVMDSDDQLRLVKR
VVQSLELDETKYPPKQMGWWINEQKDEGRRPQHIQPEPNDDWTEVRRQVYAAYQERCDRS
GLLDFAELLLRAHELLRDTPALLAHYRARFREILVDEFQDTNAIQYAFVRVLAGESGHVF
VVGDDDQAIYGWRGAKVENVQRFLKDFPGAQTVRLEQNYRSSANILGAANAVIAHNPDRI
GKQLWTDSGDGDPIDLYAAYNEVDEARYVVERARQWVRDGGSYGEVAVLYRSNAQSRALE
EALIAEQLPYRVYGGMRFFERAEIKDALAYLRMLTNRSDDAAFERAVNTPTRGIGDRTLD
EVRRLARANALSLWEAAMLCTQENTLAARARNALATFLSLVGQLQAETGEMDLAERIDHV
LMRSGLREHWAKESRGGLDSESRTENLDELVSVASRFTRPDDEDSQGMTELVAFLAYASL
EAGEGQAQAGEEGVQLMTLHSAKGLEFPIVFLVGLEDGLFPSARSLEESGRLEEERRLAY
VGITRARQKLVLCYAESRRIHGQDNYNVPSRFLREIPRDLLHEVRPKVQVSRTASLGAAR
GGPVHAVVDAAPIKLGANVEHPKFGGGVVVDYEGAGAHARVQVQFDEVGAKWLVMAYANL
TVV