Protein Info for Xcc-8004.4763.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Helicase PriA essential for oriC/DnaA-independent DNA replication

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 702 PF17764: PriA_3primeBD" amino acids 9 to 106 (98 residues), 77.1 bits, see alignment E=1.9e-25 PF04851: ResIII" amino acids 198 to 358 (161 residues), 53.3 bits, see alignment E=8e-18 PF00270: DEAD" amino acids 202 to 363 (162 residues), 66.6 bits, see alignment E=6.1e-22 TIGR00595: primosomal protein N'" amino acids 220 to 663 (444 residues), 548.9 bits, see alignment E=5.3e-169 PF18319: PriA_CRR" amino acids 444 to 475 (32 residues), 30.5 bits, see alignment (E = 6.9e-11) PF00271: Helicase_C" amino acids 499 to 591 (93 residues), 31.6 bits, see alignment E=4.2e-11

Best Hits

KEGG orthology group: K04066, primosomal protein N' (replication factor Y) (superfamily II helicase) [EC: 3.6.4.-] (inferred from 100% identity to xcb:XC_3836)

Predicted SEED Role

"Helicase PriA essential for oriC/DnaA-independent DNA replication" in subsystem Conserved gene cluster associated with Met-tRNA formyltransferase or DNA-replication

Isozymes

Compare fitness of predicted isozymes for: 3.6.4.-

Use Curated BLAST to search for 3.6.4.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H2XDG5 at UniProt or InterPro

Protein Sequence (702 amino acids)

>Xcc-8004.4763.1 Helicase PriA essential for oriC/DnaA-independent DNA replication (Xanthomonas campestris pv. campestris strain 8004)
MSAPVTTLRVALPVPLPQLFDYLPLQDTDVDGPDRVGCRVRVPFGPRELIGVVVERGQQP
SAEGLRAALDWCDDTPLLIDELARSLQWLARYTHAPLGEAQASALPGPLRRGEPLADTHA
WAWQLTEAGHTGAGSLRAGSRPALLAALLLAGPLAEEPLEQQLPQWREAARNLAKRGYAE
RVAVAADTLPARPGTGPQLNDEQQAATDAIRAGSGFATYLLDGVTGSGKTEVYLQAIADC
LAAGKQALVLVPEIGLTPQTLGRFRERLGVPVHALHSGLSDGERARVWAAAWRGEAKLIV
GTRSAVFTPLPNAGLIVIDEEHDGSYKQQDGIRYHARDFALVRGKALDVPVILGSATPSL
ESLHNAYSGRYRHLRLSRRAGDARPPRVRVLDVRKRPLKDGLSPEVLAGIGATLARGEQV
LVFKNRRGYAPVLLCHDCGWTAACQRCSTPLHQTPMTVHAGGRRLQCHHCGARQPAPLAC
PACASLALQPQGIGTERLEERLVEAFPEAPVVRIDRSTTQRRDALETQLARLGTDAGILV
GTQILAKGHDLPRLTMVVVVGIDEGLFSADFRAAEKLAQQLIQVAGRAGRADRPGEVWLQ
THHPEHPLLQTLVNGGYHAFADAELQQREAAGFPPFAHLALFRAEAKDVAAANQFLIAVR
ALVGTPATSARCCGALKRRVWRIPRSRWAARSAETTHLRTAH