Protein Info for Xcc-8004.4353.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 18 to 115 (98 residues), 101.1 bits, E=5.7e-33
• TIGR01082: UDP-N-acetylmuramate--L-alanine ligase
  • amino acids 18 to 467 (450 residues), 539 bits, E=5.4e-166
• PF08245: Mur_ligase_M
  • amino acids 120 to 299 (180 residues), 95.8 bits, E=5.4e-31
• PF02875: Mur_ligase_C
  • amino acids 334 to 458 (125 residues), 60.4 bits, E=5e-20

Best Hits

Swiss-Prot: 100% identical to MURC_XANCP: UDP-N-acetylmuramate--L-alanine ligase (murC) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K01924, UDP-N-acetylmuramate--alanine ligase [EC: 6.3.2.8] (inferred from 100% identity to xcc:XCC0726)

MetaCyc: 53% identical to UDP-N-acetylmuramate--L-alanine ligase (Escherichia coli K-12 substr. MG1655)
UDP-N-acetylmuramate--L-alanine ligase. [EC: 6.3.2.8]

Predicted SEED Role

"UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8)" in subsystem Peptidoglycan Biosynthesis (EC 6.3.2.8)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (10/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (10/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (11/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (11/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (10/17 steps found)

KEGG Metabolic Maps

• D-Glutamine and D-glutamate metabolism
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.8

Sequence Analysis Tools

See Q4UQX1 at UniProt or InterPro

Protein Sequence (477 amino acids)

>Xcc-8004.4353.1 UDP-N-acetylmuramate--alanine ligase (EC 6.3.2.8) (Xanthomonas campestris pv. campestris strain 8004)
VIRRLQDSGDLVRAFPRVHFVGIGGTGMSGIAEVMLTLGYEVSGSDNADNAATRRLAKLG
ARVMRGHSAANVLGTDCVVVSSAIREDNPELMEARSQRIPIMPRAAMLAELMRFRRGIAV
AGTHGKTTTTSLAAAVLSEGGLDPTFVIGGQLLAAGANAKLGGGQWLVAEADESDGSFLR
LNPLMAVITNIDADHLENYGNDFARVQAAFAEFLQRLPFYGLALLCIDDPEVAALAGKTP
RHVMSYGMSENADVRAEDVVQDGPRMRFTLRLPEGTTTPVTLALPGRHNVLNALAAAAIG
WQLGVAPDTIARALENFAGIGRRFNDLGEVTTASGARVRVVDDYGHHPRELEAVFAAARG
GWPDKRLVVAFQPHRYSRTRDQFDAFAAVLSTVDALVLSEVYPAGEAPIPGADSRALARA
IRARGRSEPVVVGQVAGLSEVLPDVLQDGDLLLMMGAGDIGYVAQQIVTDGFVGAPA