Protein Info for Xcc-8004.43.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Glutamate synthase [NADPH] small chain (EC 1.4.1.13)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01318: glutamate synthase, small subunit
  • amino acids 7 to 483 (477 residues), 794 bits, E=2e-243
• PF14691: Fer4_20
  • amino acids 30 to 135 (106 residues), 123.5 bits, E=1.8e-39
• PF01494: FAD_binding_3
  • amino acids 149 to 180 (32 residues), 23.6 bits, see alignment (E = 1.4e-08)
• PF07992: Pyr_redox_2
  • amino acids 149 to 470 (322 residues), 99.7 bits, E=1.1e-31
• PF13450: NAD_binding_8
  • amino acids 152 to 185 (34 residues), 28.4 bits, see alignment (E = 8e-10)
• PF00070: Pyr_redox
  • amino acids 298 to 373 (76 residues), 29.9 bits, E=3.4e-10

Best Hits

KEGG orthology group: K00266, glutamate synthase (NADPH/NADH) small chain [EC: 1.4.1.13 1.4.1.14] (inferred from 100% identity to xca:xccb100_0036)

Predicted SEED Role

"Glutamate synthase [NADPH] small chain (EC 1.4.1.13)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.1.13)

MetaCyc Pathways

• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• ammonia assimilation cycle I (2/2 steps found)
• L-glutamate biosynthesis IV (1/1 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-glutamine degradation II (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of ammonia assimilation (plants) (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Glutamate metabolism
• Nitrogen metabolism

Isozymes

Compare fitness of predicted isozymes for: 1.4.1.13

Use Curated BLAST to search for 1.4.1.13 or 1.4.1.14

Sequence Analysis Tools

See A0A0H2X3N0 at UniProt or InterPro

Protein Sequence (493 amino acids)

>Xcc-8004.43.1 Glutamate synthase [NADPH] small chain (EC 1.4.1.13) (Xanthomonas campestris pv. campestris strain 8004)
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQSGRCLDCGNPYCSWK
CPVHNAIPQWLQLVQEDRIIEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
GAVEKYIVDTAFSMGWRPDLGNVQPSGHRVAVVGAGPAGLSCADRLVRAGIEAVVYDRYE
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
GAYRYTDGGLDGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQIALPDLNGKRVV
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDESSMPGSAREVANAREEGVRFLFNRQPLSI
ESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPTVPEWLSS
QGVEAGSNGRIVAPAEGNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
LGVKAQVKEPAAA