Protein Info for Xcc-8004.3822.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Isoleucyl-tRNA synthetase (EC 6.1.1.5)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 943 TIGR00392: isoleucine--tRNA ligase" amino acids 16 to 858 (843 residues), 887.5 bits, see alignment E=5.1e-271 PF00133: tRNA-synt_1" amino acids 29 to 657 (629 residues), 468.8 bits, see alignment E=3.6e-144 PF09334: tRNA-synt_1g" amino acids 59 to 202 (144 residues), 37.9 bits, see alignment E=2.5e-13 amino acids 598 to 655 (58 residues), 20.2 bits, see alignment 5.6e-08 PF08264: Anticodon_1" amino acids 701 to 857 (157 residues), 103 bits, see alignment E=3.7e-33 PF06827: zf-FPG_IleRS" amino acids 905 to 932 (28 residues), 32.9 bits, see alignment (E = 1.1e-11)

Best Hits

Swiss-Prot: 100% identical to SYI_XANCP: Isoleucine--tRNA ligase (ileS) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K01870, isoleucyl-tRNA synthetase [EC: 6.1.1.5] (inferred from 94% identity to xac:XAC1254)

Predicted SEED Role

"Isoleucyl-tRNA synthetase (EC 6.1.1.5)" (EC 6.1.1.5)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4US40 at UniProt or InterPro

Protein Sequence (943 amino acids)

>Xcc-8004.3822.1 Isoleucyl-tRNA synthetase (EC 6.1.1.5) (Xanthomonas campestris pv. campestris strain 8004)
VTQDYKATLHLPATDFPMRGDLPKREPAMLERWEREGLYAQVRANAAGRPLFVLHDGPPY
ANGQIHLGHAVNKILKDIIVKSKYLAGFDAPYIPGWDCHGLPIEIAIEKKFGKVGVKLDA
AQFRQKCREYATEQIDLQRRDFKRLGVIGDWDNPYKTLDFRFEANEIRALAKVIDNGHLT
RGVKPVHWCFDCGSALAEAEIEYADKLSPTVDIAYPARDPAAIAAAFGVTLPAGTQVAVP
IWTTTPWTLPASLAVSLGAELDYVLVEGPADRGQPRWLVIAEALAGKALARYGVEAVVVH
GHAKGAALDQLLLAHPFYAERDIPLILGDHVSDDDGTGAVHTAPGHGQEDYQVSKQYGLL
ERYSAAQINPIDGRGVYLPSTPPLGDTVLAGLHIWKANDVIIDALRDTGVLLAASTMEHS
YPHCWRHKTPIAFRATPQWFISMEQANLRADALKAIETVHWYPSWGQARIAGMIDGRPDW
TISRQRTWGVPIALFVHRETGEPHPRSTELMRQVAERVEQGGVDVWYTLDAAELLGDEAG
DYDKITDILDVWFDSGVTHEAVLVDRGLPKPADLYLEGSDQHRGWFQSSLLTGVAMDKVA
PYKQCLTHGFTVDEHGRKMSKSLGNGIEPQEIMRTLGADILRLWIASADYSNEMSLSQEI
LKRNADAYRRLRNTARFLLGNLHGFDPLQHLVALDDMVLLDRWIVHRAHELQEKITAAYA
RYDFAEIVQALLNFCSVDLGSLYLDVTKDRLYTMAEDARGRRSAQSAMYHVAEAFVRWIA
PVLSFTAEELWAYLPGEHSGNVLFATWYDGLAPMPADAALTSADVDKLLALREQVAKVLE
PMRANGAIGAALEAEITVAADAQTAARWQPLSDELRFLFISGDVTVTAASTDDIFVSAQP
TTKAKCVRCWHHQASVGSDPRHPELCSRCVSNIEGPGEERRWF