Protein Info for Xcc-8004.3688.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02771: Acyl-CoA_dh_N
  • amino acids 15 to 125 (111 residues), 113.5 bits, E=1.4e-36
• PF02770: Acyl-CoA_dh_M
  • amino acids 131 to 224 (94 residues), 94.9 bits, E=5.2e-31
• PF00441: Acyl-CoA_dh_1
  • amino acids 236 to 386 (151 residues), 174.7 bits, E=2.9e-55
• PF08028: Acyl-CoA_dh_2
  • amino acids 253 to 374 (122 residues), 82.3 bits, E=7.6e-27

Best Hits

Swiss-Prot: 56% identical to ACAD8_DICDI: Isobutyryl-CoA dehydrogenase, mitochondrial (acad8) from Dictyostelium discoideum

KEGG orthology group: K00257, [EC: 1.3.99.-] (inferred from 100% identity to xca:xccb100_3042)

MetaCyc: 46% identical to short-chain acyl-CoA dehydrogenase monomer (Pseudomonas putida KT2440)
RXN-13449 [EC: 1.3.8.1]

Predicted SEED Role

"Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12)" in subsystem Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Leucine Degradation and HMG-CoA Metabolism or Valine degradation (EC 1.3.99.12)

MetaCyc Pathways

• oleate β-oxidation (31/35 steps found)
• superpathway of coenzyme A biosynthesis II (plants) (7/10 steps found)
• β-alanine biosynthesis II (3/6 steps found)
• valproate β-oxidation (5/9 steps found)

KEGG Metabolic Maps

• 1- and 2-Methylnaphthalene degradation
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Brassinosteroid biosynthesis
• Caprolactam degradation
• Fatty acid metabolism
• Geraniol degradation
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.-

Use Curated BLAST to search for 1.3.8.1 or 1.3.99.- or 1.3.99.12

Sequence Analysis Tools

See A0A0H2X9M0 at UniProt or InterPro

Protein Sequence (393 amino acids)

>Xcc-8004.3688.1 Branched-chain acyl-CoA dehydrogenase (EC 1.3.99.12) (Xanthomonas campestris pv. campestris strain 8004)
MNAAIQLPTDPVDLNDEQQAFRAAARDFADKELAPHAARWDAESHFPREAIGKAAELGFC
GLYTDEGVGGLGMRRLDAAIVFEELATVDPSTSAFITIHNMATWLIASYGNDGVRAQWGE
AMTSGAKLGSYCLTEPGSGSDAASLKTRAQRDGDDYVLNGSKAFISGAGATDVLVVMART
GEDGARGISAFVVAADAPGISYGRKEEKMGWNSQPTRGITFTDVRIPAGNLLGKEGEGFK
MAMKALDGGRINIAACSLGAAQGALDAARRYMGERRQFGKKLADFQALQFKLADMATQLV
AARQMVHTAARKLDAGSHDATVWCAMAKRFATDAGFAICDEALQIHGGYGYIREYPIERL
LRDSRVHRILEGTNEVMRMIIARHLLNSEEQLR