Protein Info for Xcc-8004.351.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: tRNA pseudouridine synthase A (EC 4.2.1.70)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00849: PseudoU_synth_2
  • amino acids 2 to 148 (147 residues), 77.3 bits, E=7.7e-26
• TIGR00093: pseudouridine synthase
  • amino acids 6 to 176 (171 residues), 169.1 bits, E=3.4e-54

Best Hits

Swiss-Prot: 100% identical to RLUE_XANCP: Ribosomal large subunit pseudouridine synthase E (rluE) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K06181, ribosomal large subunit pseudouridine synthase E [EC: 5.4.99.12] (inferred from 100% identity to xca:xccb100_0298)

Predicted SEED Role

"tRNA pseudouridine synthase A (EC 4.2.1.70)" in subsystem tRNA processing (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Sequence Analysis Tools

See A0A0H2X2Y4 at UniProt or InterPro

Protein Sequence (187 amino acids)

>Xcc-8004.351.1 tRNA pseudouridine synthase A (EC 4.2.1.70) (Xanthomonas campestris pv. campestris strain 8004)
MLVLLNKPYGVLSQFSDRSTPPKRTLAEFGLPPQVYAAGRLDHDSEGLLLLTDDGPLAHR
LTDPRHKQPKTYWVQVEGDPDTSQLQALCDGVLLNDGPTRPANVRRLECAPTLWPRDPPV
RVRKTVPDAWLEVQITEGRNRQVRRMTASVGLPTLRLVRVAIGAWSLASLQPGQWRVDDA
RAVRPAR