Protein Info for Xcc-8004.3485.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Replicative DNA helicase (EC 3.6.1.-)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 TIGR00665: replicative DNA helicase" amino acids 33 to 468 (436 residues), 588.2 bits, see alignment E=4.9e-181 PF00772: DnaB" amino acids 33 to 134 (102 residues), 124.2 bits, see alignment E=3.3e-40 PF03796: DnaB_C" amino acids 211 to 466 (256 residues), 377.7 bits, see alignment E=3.9e-117 PF13481: AAA_25" amino acids 229 to 388 (160 residues), 44.7 bits, see alignment E=1.8e-15

Best Hits

Swiss-Prot: 49% identical to DNAC_BACSU: Replicative DNA helicase (dnaC) from Bacillus subtilis (strain 168)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 100% identity to xca:xccb100_2864)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-)" in subsystem DNA-replication (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H2X8W5 at UniProt or InterPro

Protein Sequence (476 amino acids)

>Xcc-8004.3485.1 Replicative DNA helicase (EC 3.6.1.-) (Xanthomonas campestris pv. campestris strain 8004)
MSARPGFRSNRNRDRDRDDYDRPEPRLDQLRVPPHSVEAEQAVLGGLMLAPDAFDKVNDQ
LTENDFYRRDHRLIYRAIRELSEKDRPFDAVTLGEWFESQGKLEQVGDGAYLIELASTTP
SAANIAAYAEIVRDKAVLRQLIEVGTNIVNDGFQPEGRESVELLASAEKAVFKIAEAGAR
GRTDFVAMPGALKDAFEELRNRFENGGNITGLPTGYTDFDAMTAGLQPTDLIILAARPAM
GKTTLALNIAEYAAIKSKKGVAVFSMEMSASQLAMRLISSNGRINAQRLRTGALEDEDWA
RVTGAIKMLKETKIFIDDTPGVSPEVLRSKCRRLKREHDLGLIVIDYLQLMSVPGNSENR
ATEISEISRGLKGLAKELNVPVIALSQLNRSLETRTDKRPVMADLRESGAIEQDADMIVF
IYRDDYYNKENSPDKGLAEIIIGKHRGGPTGSCKLKFFGEYTRFDNLAHDSVGSFE