Protein Info for Xcc-8004.310.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Malate synthase (EC 2.3.3.9)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF20656: MS_N
  • amino acids 29 to 78 (50 residues), 38.5 bits, 1.3e-13
• TIGR01344: malate synthase A
  • amino acids 33 to 539 (507 residues), 712.8 bits, E=8.9e-219
• PF01274: MS_TIM-barrel
  • amino acids 172 to 416 (245 residues), 354 bits, E=6.6e-110
• PF20659: MS_C
  • amino acids 422 to 538 (117 residues), 115.6 bits, E=3.5e-37

Best Hits

Swiss-Prot: 58% identical to MASY_MYXXD: Malate synthase (mls) from Myxococcus xanthus (strain DK 1622)

KEGG orthology group: K01638, malate synthase [EC: 2.3.3.9] (inferred from 100% identity to xcb:XC_0247)

MetaCyc: 54% identical to malate synthase A (Escherichia coli K-12 substr. MG1655)
Malate synthase. [EC: 2.3.3.9]

Predicted SEED Role

"Malate synthase (EC 2.3.3.9)" in subsystem Photorespiration (oxidative C2 cycle) (EC 2.3.3.9)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (24/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• glyoxylate cycle (6/6 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• glycolate and glyoxylate degradation II (1/2 steps found)
• chitin deacetylation (2/4 steps found)
• superpathway of glycol metabolism and degradation (3/7 steps found)
• L-arabinose degradation IV (3/8 steps found)
• D-xylose degradation IV (2/7 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (1/14 steps found)
• superpathway of pentose and pentitol degradation (9/42 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.3.3.9

Sequence Analysis Tools

See Q4V039 at UniProt or InterPro

Protein Sequence (540 amino acids)

>Xcc-8004.310.1 Malate synthase (EC 2.3.3.9) (Xanthomonas campestris pv. campestris strain 8004)
MSATAASARPVAADRATPGVSLTTQLAGQAELLPAAALALLVSLHRAIEPGRQQRLAQRR
SRQAAFDAGQLPDFRTDTRAIRASDWRVAELPAALQDRRIEITGPTDPKMVINALNSGAK
VFMADFEDSTAPTWRNLLAGQRTLAAAVRGELTFDAPNGKHYALRPEHERAVLIVRPRGW
HLDEKHVLIDGQPMAGGLFDAALFAFHNARTLMAKDRGPYLYLPKLQSMEEAALWETALA
HIEAMLGLPHGQIKVTVLIETLPAVFEMDEILHALRARIVGLNCGRWDYIFSYLKTFRAH
PDRVLPERGQVTMTQPFLKAYSELLIKTCHRRGAHAMGGMAAQIPINHDEAANEQAMARV
RADKLREVSAGHDGTWVAHPALIPVAMAVFDEHMPTAHQHHVLRNDVHITSDMLIATPPG
GITRAGFEGNVEVCVRYLAAWLDGNGCVPIHNLMEDAATAEISRAQLWQWLHHGRHLDDG
TAIDQHLLDTTLRQLPARLGTTAGVPGGARIAEAIGLLDQLSRAEELADFLTVPAYRLID