Protein Info for Xcc-8004.2393.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00888: GMP synthase (glutamine-hydrolyzing), N-terminal domain
  • amino acids 9 to 200 (192 residues), 248 bits, E=4.6e-78
• PF00117: GATase
  • amino acids 10 to 196 (187 residues), 150 bits, E=2e-47
• PF07722: Peptidase_C26
  • amino acids 73 to 179 (107 residues), 42.3 bits, E=2.3e-14
• TIGR00884: GMP synthase (glutamine-hydrolyzing), C-terminal domain
  • amino acids 208 to 521 (314 residues), 497.6 bits, E=1.4e-153
• PF02540: NAD_synthase
  • amino acids 214 to 296 (83 residues), 33.7 bits, E=6.5e-12
• PF03054: tRNA_Me_trans
  • amino acids 225 to 290 (66 residues), 21.4 bits, E=5.4e-08
• PF00958: GMP_synt_C
  • amino acids 429 to 520 (92 residues), 142.9 bits, E=8e-46

Best Hits

Swiss-Prot: 100% identical to GUAA_XANC8: GMP synthase [glutamine-hydrolyzing] (guaA) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K01951, GMP synthase (glutamine-hydrolysing) [EC: 6.3.5.2] (inferred from 100% identity to xcb:XC_1935)

MetaCyc: 69% identical to GMP synthetase (Escherichia coli K-12 substr. MG1655)
GMP synthase (glutamine-hydrolyzing). [EC: 6.3.5.2]; 6.3.5.2 [EC: 6.3.5.2]

Predicted SEED Role

"GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2)" in subsystem Purine conversions or Staphylococcal pathogenicity islands SaPI (EC 6.3.5.2)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (44/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (24/26 steps found)
• superpathway of purine nucleotide salvage (12/14 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (7/8 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• ammonia assimilation cycle III (3/3 steps found)
• L-glutamate biosynthesis I (2/2 steps found)
• L-glutamine degradation I (1/1 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-asparagine biosynthesis III (tRNA-dependent) (3/4 steps found)
• glutaminyl-tRNA^gln biosynthesis via transamidation (3/4 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-glutamate and L-glutamine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Drug metabolism - other enzymes
• Glutamate metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.5.2

Use Curated BLAST to search for 6.3.5.2

Sequence Analysis Tools

See Q4UVC5 at UniProt or InterPro

Protein Sequence (521 amino acids)

>Xcc-8004.2393.1 GMP synthase [glutamine-hydrolyzing] (EC 6.3.5.2) (Xanthomonas campestris pv. campestris strain 8004)
MSSLHNDKILILDFGAQYTQLIARRIREIGVYCEIWAWDHDPSEIAGFGAKGIILSGGPE
STTLPGAPVAPQEVFDSGLPVFGICYGMQTLAAQLGGATEAADQREFGHAEVDVVAADAL
FSGLTDHAGASRLNVWMSHGDHVSQVPPGFTITAVTDRIPVAAMSNEDKRWYGVQFHPEV
THTLQGQTLLRRFVVDVCGCQTLWTAANIIDDQIARVREQVGDDEVILGLSGGVDSSVVA
ALLHKAIGDKLTCVFVDTGLLRWQEGDQVMAMFAEHMGVKVIRVNAADRYFAKLEGVSDP
EAKRKIIGNLFVDIFDEESNKLANAKWLAQGTIYPDVIESAGSKTGKAHVIKSHHNVGGL
PEHMKLGLVEPLRELFKDEVRRLGVELGLPRTMVYRHPFPGPGLGVRILGEVKREYAELL
AKADAIFIDELRKADLYDKTSQAFAVFLPVKSVGVVGDARAYEWVIALRAVETIDFMTAH
WAHLPYDFLGTVSNRIINELRGVSRVVYDISGKPPATIEWE