Protein Info for Xcc-8004.2067.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Threonyl-tRNA synthetase (EC 6.1.1.3)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 636 PF02824: TGS" amino acids 4 to 63 (60 residues), 48.1 bits, see alignment 2e-16 TIGR00418: threonine--tRNA ligase" amino acids 74 to 633 (560 residues), 714.3 bits, see alignment E=7e-219 PF07973: tRNA_SAD" amino acids 174 to 221 (48 residues), 56.5 bits, see alignment 4.5e-19 PF00587: tRNA-synt_2b" amino acids 322 to 531 (210 residues), 156.2 bits, see alignment E=1.9e-49 PF03129: HGTP_anticodon" amino acids 543 to 632 (90 residues), 79.5 bits, see alignment E=3.4e-26

Best Hits

Swiss-Prot: 100% identical to SYT_XANCB: Threonine--tRNA ligase (thrS) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to xca:xccb100_1704)

MetaCyc: 62% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4UW57 at UniProt or InterPro

Protein Sequence (636 amino acids)

>Xcc-8004.2067.1 Threonyl-tRNA synthetase (EC 6.1.1.3) (Xanthomonas campestris pv. campestris strain 8004)
LPMITITLPDGSRREFDAPVSVMQVAQSIGAGLAKATIAGQVDGQLVDASDLIEHDASLR
IITAKDAEGVEIIRHSCAHLVGHAVKQLYPDVKMVIGPVIAEGFYYDIYSERPFTPEDMA
AIEQRMQQLIAQDYDVIKKVTPRAEVIEVFAQRGEEYKLRLIEDMSDDITAMGLYYHQEY
VDMCRGPHVPNTRFLKAFKLTRISGAYWRGDAKNEQLQRIYGTAWADKKQLDAYILRMEE
ADKRDHRKIGKAQDLFHLQEEAPGLVFWHPKGWSLWQVVEQYMRKVYRDSGYGEVRCPQI
LDVSLWQKSGHWDNYQDAMFFTESEKRTYAVKPMNCPGHVQVFNQGLHSYRDLPIRYGEF
GACHRNEPSGALHGILRVRGFTQDDGHVFCLESQIEAEVTAFHQQALAVYTAFGFDDIQI
KIALRPEKRLGDDATWDKAEAALRSALGVCGVEWQELPGEGAFYGPKIEYHLKDAIGRTW
QLGTMQVDFMMPGRLGAEYVDEHSQKKHPVMLHRAIVGSMERFIGILIEHHAGAFPAWLA
PVQVVVANITDAQAEYVDSVRKTLANQGFRVSADLRNEKIGYKIREHTLQRVPYLLVVGD
REKENGAVAVRTRSGEDLGTMTVSAFIERLQAEQAA