Protein Info for Xcc-8004.1953.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 308 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 9 to 290 (282 residues), 387.7 bits, see alignment E=3.1e-120 TIGR00536: methyltransferase, HemK family" amino acids 11 to 287 (277 residues), 235.9 bits, see alignment E=5e-74 PF06325: PrmA" amino acids 131 to 203 (73 residues), 22.4 bits, see alignment E=1.9e-08 PF05175: MTS" amino acids 131 to 212 (82 residues), 54.1 bits, see alignment E=3.7e-18 PF13847: Methyltransf_31" amino acids 131 to 260 (130 residues), 35.7 bits, see alignment E=1.8e-12 PF13649: Methyltransf_25" amino acids 134 to 214 (81 residues), 31 bits, see alignment E=8.5e-11 PF08242: Methyltransf_12" amino acids 134 to 203 (70 residues), 30.4 bits, see alignment E=1.4e-10

Best Hits

Swiss-Prot: 100% identical to PRMB_XANCP: 50S ribosomal protein L3 glutamine methyltransferase (prmB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 100% identity to xcc:XCC2553)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See A0A0H2X7T3 at UniProt or InterPro

Protein Sequence (308 amino acids)

>Xcc-8004.1953.1 Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p (Xanthomonas campestris pv. campestris strain 8004)
MTAAAADELHTIIDLIRYGTSRFNEAGLTFGHSYDNALDEATQLVLHSLHLPHDLGPAYG
QARLLRAEKERVLALFQRRVDERVPAAYLTGEAWFAGLSFKSDARALVPRSPIAELIEAG
FEPWLGGREVTRALDLCTGSGCIAIAMGHYNPHWDVDGVDISDDALALAAENKARLHADN
VSLLKSDLFTGLAGRQYDLIVTNPPYVTNDETDALPQEYSYEPELGLRAGDDGLDLVLKI
LRDAPQHLSEDGLLICEVGESEQHLIKLLPEVDFAWVEFKVGQMGIFAVECRELIAHSAR
ITELASAR