Protein Info for Xcc-8004.186.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 731 TIGR01515: 1,4-alpha-glucan branching enzyme" amino acids 111 to 727 (617 residues), 917 bits, see alignment E=2.9e-280 PF02922: CBM_48" amino acids 127 to 210 (84 residues), 83.2 bits, see alignment E=2e-27 PF00128: Alpha-amylase" amino acids 269 to 350 (82 residues), 35.5 bits, see alignment E=1.3e-12 PF02806: Alpha-amylase_C" amino acids 631 to 729 (99 residues), 85.6 bits, see alignment E=3.6e-28

Best Hits

Swiss-Prot: 100% identical to GLGB1_XANCP: 1,4-alpha-glucan branching enzyme GlgB 1 (glgB1) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: K00700, 1,4-alpha-glucan branching enzyme [EC: 2.4.1.18] (inferred from 100% identity to xcb:XC_0143)

Predicted SEED Role

"1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18)" in subsystem Glycogen metabolism or Trehalose Biosynthesis (EC 2.4.1.18)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.4.1.18

Use Curated BLAST to search for 2.4.1.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4V0E2 at UniProt or InterPro

Protein Sequence (731 amino acids)

>Xcc-8004.186.1 1,4-alpha-glucan (glycogen) branching enzyme, GH-13-type (EC 2.4.1.18) (Xanthomonas campestris pv. campestris strain 8004)
MSGVMTAVTNRWDPGVTRALAEARHGDAFAVLGAHPSTNGRLLRTYHPGAEHVTAVLADG
REVPLEAGPEPGLFAGELPAEGRYRLRIGWPGGTQDTADPYAFGPLLSDFDLHLISEGHH
LQLADALGANAVEVDGVRGTRFAVWAPNASRVAVVGDFNSWDARRHPMRLRHQAGVWELF
VPDVGPGAHYKYQLRGPHGHELPAKADPVARRAELAPGTASIVADPTPHQWSDDGWMATR
ARRQAHDAPMSIYEIHAGSWLREEGLDLDWDGLADRLIPYVADMGFTHVELMPVTEHPFG
GSWGYQPLGLFAPTARFGSPDGFARFVDRCHREGIGVIVDWVPAHFPTDAHGLAHFDGTA
LYEHADPREGFHRDWNTLIYNHGRREVSGFLIASALEFLQRYHVDGLRVDAVASMLYRDY
SRNAGEWVPNIHGGRENYETIAFLRRLNEVVREHAPGAVTIAEESTAWPGVTADVSHGGL
GFHYKWNMGWMHDTLHYIGLDPIYRRYHHGELTFSMVYAYSERFVLPISHDEVVHGKGSL
LGRMPGDDWQRFANLRAYLGFMFTHPGRKLLFMGCEFGQPTEWNHDAGLPWHLLDDARHR
GVQTLVRDLNHLYAQYPALHAHDDDPSGFAWLVGDDAANSVVAFLRKGKRGDAPVLVVIN
YTPVVQQGYRLGVPQGGLWREVFNSDAGIYGGANLGNGGAVTAEPQSMHGHAQSLPLLLP
PLGVIVLAPQG