Protein Info for Xcc-8004.1789.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Glutamyl-tRNA synthetase (EC 6.1.1.17)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 100% identical to SYE_XANC8: Glutamate--tRNA ligase (gltX) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K01885, glutamyl-tRNA synthetase [EC: 6.1.1.17] (inferred from 100% identity to xca:xccb100_1475)

MetaCyc: 55% identical to glutamate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Glutamate--tRNA ligase. [EC: 6.1.1.17]

Predicted SEED Role

"Glutamyl-tRNA synthetase (EC 6.1.1.17)" in subsystem Heme and Siroheme Biosynthesis or tRNA aminoacylation, Glu and Gln (EC 6.1.1.17)

MetaCyc Pathways

tRNA charging (21/21 steps found)
superpathway of heme b biosynthesis from glutamate (9/10 steps found)
tetrapyrrole biosynthesis I (from glutamate) (6/6 steps found)
adenosylcobalamin biosynthesis II (aerobic) (21/33 steps found)
(2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
adenosylcobalamin biosynthesis I (anaerobic) (20/36 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.17

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4UWS6 at UniProt or InterPro

Protein Sequence (467 amino acids)

>Xcc-8004.1789.1 Glutamyl-tRNA synthetase (EC 6.1.1.17) (Xanthomonas campestris pv. campestris strain 8004)
MTCRTRFAPSPTGYLHIGGARTALYCWLEARHRGGQFVLRIEDTDRERSTQVAIDAILEA
MEWLGLGYDEGPIYQTQRIARYQEVAEQLLAQGKAYYAYETREELDAMREAAMAKQEKPR
YNGAAREQQLPYRDDPNRVIRFKNPLAGTVVFDDLIKGRIEIANSELDDMVIFRPDGYPT
YNFAVVVDDWDMGITEVIRGDDHINNTPRQINIYEALGAPVPKFAHMPMILDEQGAKLSK
RTGAADVMQYKDAGYLPHALINYLARLGWSHGDQELFSQQELLDLFDVKDVNSKAARLDM
AKLGWVNQHYLKTDDPASIAPQLEYQLRKLGIDVAAGPAAADVVVALRERVQTLKEMAEK
AVVWYQPLETYDEAAVIKHLKLGAEVPLGKAREMLAALNEWSVENVSAALHAAAAALELG
MGKVAQPLRVAITGTQVSPDISQTVYLAGREGALKRIDAALIKIGAA