Protein Info for Xcc-8004.1431.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Aspartyl-tRNA synthetase (EC 6.1.1.12)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 588 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 583 (583 residues), 751.3 bits, see alignment E=3.4e-230 PF01336: tRNA_anti-codon" amino acids 18 to 105 (88 residues), 53.6 bits, see alignment E=2.6e-18 PF00152: tRNA-synt_2" amino acids 121 to 558 (438 residues), 392.7 bits, see alignment E=1.9e-121 PF02938: GAD" amino acids 311 to 405 (95 residues), 84.9 bits, see alignment E=5.7e-28

Best Hits

Swiss-Prot: 100% identical to SYD_XANC8: Aspartate--tRNA ligase (aspS) from Xanthomonas campestris pv. campestris (strain 8004)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 100% identity to xca:xccb100_1166)

MetaCyc: 56% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12)" in subsystem tRNA aminoacylation, Asp and Asn (EC 6.1.1.12)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4UXM5 at UniProt or InterPro

Protein Sequence (588 amino acids)

>Xcc-8004.1431.1 Aspartyl-tRNA synthetase (EC 6.1.1.12) (Xanthomonas campestris pv. campestris strain 8004)
MRTHFCGLVDETLIGQTVTLAGWTDVARNLGGVCFIDLRDHEGIVQVTVEPAEGDANSAE
VFKVAASLGYEDVLQVEGVVRARHAVNDKIRTGKVEVIATRITILNKAAPLPFHAHENPG
EDTRLKYRYLDLRRPEMQRMQRTRIKLVQALRRHLDARDFQDIETPILTKATPEGARDFL
VPARMHPGEFYALPQSPQLFKQILMVAGFDRYYQIARCFRDEALRADRQLEFTQLDMEFA
FVRERDVQDFVEDMIRAIFKEVVDVELAAQFPRMTWAEAMRRYGSDKPDLRIALELVDVA
ELVKTSEFPVFAAAANDADGRVAALRIPGGATLSRKQIDDYAAHAAKYGAKGLAYSKLSE
TGEVSSPIAKFFGEEAFAALLAHVGAANGDIVFFGAGSYNKVSDFMGALRLKAGKDFGLV
AAGWAPLWVTDFPMFEWDEEAQRYVALHHPFTAPAVDDIADLRANARTAVSRGYDMVLNG
NEIGGGSIRIHRPDMQSAVFELLGIGAEEARAKFGFLLDALNYGAPPHGGIAFGIDRIAA
LMAGTESIRDVIPFPKTTGAQDLMTDAPSPIAAEQLAEVHVQVRPKQA