Protein Info for Xcc-8004.1221.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: Ribonuclease PH (EC 2.7.7.56)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 241 TIGR01966: ribonuclease PH" amino acids 5 to 239 (235 residues), 383.7 bits, see alignment E=1.3e-119 PF01138: RNase_PH" amino acids 13 to 143 (131 residues), 100.7 bits, see alignment E=8.9e-33 PF03725: RNase_PH_C" amino acids 161 to 227 (67 residues), 48.9 bits, see alignment E=5e-17

Best Hits

Swiss-Prot: 100% identical to RNPH_XANCB: Ribonuclease PH (rph) from Xanthomonas campestris pv. campestris (strain B100)

KEGG orthology group: K00989, ribonuclease PH [EC: 2.7.7.56] (inferred from 100% identity to xcb:XC_0960)

Predicted SEED Role

"Ribonuclease PH (EC 2.7.7.56)" in subsystem Heat shock dnaK gene cluster extended or tRNA processing (EC 2.7.7.56)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.56

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q4UY39 at UniProt or InterPro

Protein Sequence (241 amino acids)

>Xcc-8004.1221.1 Ribonuclease PH (EC 2.7.7.56) (Xanthomonas campestris pv. campestris strain 8004)
MSFSRPSGRTADQLRPVRIERAFTRHAEGSVLVSFGDTRVLCTASVENRVPNFLRGKGEG
WVTAEYGMLPRSTHTRSDREAARGKQGGRTLEIQRLIGRALRACVDRNALGERTITLDCD
VLQADGGTRTAAITGAYVALADAVNLLLKRGDIKKHPLIGAVAAVSVGIYRGEPVLDLDY
PEDSDCDTDMNVVMNDGGGFIELQGTAEGHAFRRDELNALLALAEKGVGELFELQRAALA
G