Protein Info for Xcc-8004.1035.1 in Xanthomonas campestris pv. campestris strain 8004

Annotation: NADP-dependent malic enzyme (EC 1.1.1.40)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00390: malic
  • amino acids 20 to 152 (133 residues), 94 bits, E=1.5e-30
• PF03949: Malic_M
  • amino acids 166 to 360 (195 residues), 73.6 bits, E=2.6e-24
• PF01515: PTA_PTB
  • amino acids 428 to 748 (321 residues), 316.9 bits, E=2.7e-98

Best Hits

Swiss-Prot: 59% identical to MAO2_SALTY: NADP-dependent malic enzyme (maeB) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K00029, malate dehydrogenase (oxaloacetate-decarboxylating)(NADP+) [EC: 1.1.1.40] (inferred from 100% identity to xcb:XC_0821)

Predicted SEED Role

"NADP-dependent malic enzyme (EC 1.1.1.40)" in subsystem Pyruvate metabolism I: anaplerotic reactions, PEP (EC 1.1.1.40)

MetaCyc Pathways

• gluconeogenesis I (11/13 steps found)
• methylgallate degradation (5/6 steps found)
• protocatechuate degradation I (meta-cleavage pathway) (6/8 steps found)
• C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
• superpathway of vanillin and vanillate degradation (6/10 steps found)
• syringate degradation (7/12 steps found)
• C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of terpenoids and steroids
• Carbon fixation in photosynthetic organisms
• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.40

Sequence Analysis Tools

See A0A0H2X5W0 at UniProt or InterPro

Protein Sequence (769 amino acids)

>Xcc-8004.1035.1 NADP-dependent malic enzyme (EC 1.1.1.40) (Xanthomonas campestris pv. campestris strain 8004)
MSNDDFKQAALDYHRQQPAGKIKVTATKPMLTQRDLSLAYSPGVAFACEAIVEDATQASE
LTARGNLVAVITNGTAVLGLGNIGPLASKPVMEGKGVLFQKFAGIDVFDIEINENDPDKL
VDIIASLEPTFGGINLEDIKAPECFIVERKLRERMNIPVFHDDQHGTAIIVGAAVLNALV
VTGKKIEDVKLATTGMGAAGISCVNMLVSLGLKSENILALDRDGVIHTGRTDLDPDKQRY
ARDTDKRTLAEIVEGADIFLGLSAAGILKPEMVATMARQPVIFALANPNPEITPEAAKAV
RPDCIIGTGRSDYPNQINNVLCFPYLFRGALDVGATGINEDMKIACVKAIAAMARREASD
LGAAYGGETPSFGPEYLIPRPLDPRLLVELSSAVAQAAMDSGVATRPIADMAAYRDRLSQ
FVYRTSLMMKPVYDRARADKQRVVYAEGEEEVVLRAVQNVIDEGLALPILIGRPDVIEAR
IQRMGLRIRAGEDFEITNIHDDPRFNDYWQYYYALTERRGVTVTAAKELMRSRPTLIAAV
MVARGEADAMLSGVVGRFHKKLGYARSVIPLEPRVSSTSAMTGVINQQGVFFFLDTHVQE
DPTVEQVVEATLQAAYRLKLFGIEPNIALLSHSNFGSHDSRDALKMREVREALLKRKPEL
NIDGEMQGDTAWDEALRKQIMPNSTLKGRANLFVLPNLEAANIAYNLVRVFTDGVAIGPI
LMGISKPVHILTTSATSRRVMNMTAIAAVDAQIRKQRDAEKHNAEKNGG