Protein Info for TX73_024435 in Rhodopseudomonas palustris CGA009

Annotation: Gfo/Idh/MocA family oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 319 PF01408: GFO_IDH_MocA" amino acids 5 to 124 (120 residues), 83 bits, see alignment E=2.8e-27 PF19858: OxRdtase_C" amino acids 154 to 316 (163 residues), 319.4 bits, see alignment E=3.3e-100

Best Hits

Swiss-Prot: 79% identical to LIGC_SPHSK: 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase (ligC) from Sphingobium sp. (strain NBRC 103272 / SYK-6)

KEGG orthology group: K10219, 2-hydroxy-4-carboxymuconate semialdehyde hemiacetal dehydrogenase [EC: 1.1.1.312] (inferred from 100% identity to rpt:Rpal_5184)

MetaCyc: 86% identical to alpha-hydroxy-gamma-carboxymuconate epsilon-semialdehyde dehydrogenase subunit (Pseudomonas straminea)
1.2.1.45-RXN [EC: 1.1.1.312]

Predicted SEED Role

"4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase"

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.1.312

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (319 amino acids)

>TX73_024435 Gfo/Idh/MocA family oxidoreductase (Rhodopseudomonas palustris CGA009)
MAKTIKVALAGAGAFGIKHLDGIKNIDGVEVVSLIGRRLEPTQEVAKKYGIAHVSTELSD
ALALKEVDAVILCTPTQMHAQQGIDCLRAGKHVQVEIPLADSLKDAQAVVDLQKETGLVA
MCGHTRRFNPSHQYVHKKIKAGELNVQQMDVQTYFFRRTNMNALGQPRSWTDHLLWHHAA
HTVDLFAYQAGSPIVKANAVQGPIHPDLGIAMDMSIQLKAANGAICTLSLSFNNDGPLGT
FFRYICDNGTYIARYDDLVNGKEEKIDVSKVDVSMNGIELQDREFFAAIREGREPNSSVA
QVLPCYQVLHDLDQQLAEG