Protein Info for TX73_024250 in Rhodopseudomonas palustris CGA009

Annotation: 2-isopropylmalate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 553 TIGR00970: 2-isopropylmalate synthase" amino acids 5 to 553 (549 residues), 797.1 bits, see alignment E=4.3e-244 PF00682: HMGL-like" amino acids 32 to 312 (281 residues), 233.6 bits, see alignment E=3e-73 PF08502: LeuA_dimer" amino acids 424 to 552 (129 residues), 81.1 bits, see alignment E=6.9e-27

Best Hits

Swiss-Prot: 64% identical to LEU1_PSE14: 2-isopropylmalate synthase (leuA) from Pseudomonas savastanoi pv. phaseolicola (strain 1448A / Race 6)

KEGG orthology group: K01649, 2-isopropylmalate synthase [EC: 2.3.3.13] (inferred from 100% identity to rpa:RPA4665)

Predicted SEED Role

"2-isopropylmalate synthase (EC 2.3.3.13)" in subsystem Branched-Chain Amino Acid Biosynthesis or Leucine Biosynthesis (EC 2.3.3.13)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.3.3.13

Use Curated BLAST to search for 2.3.3.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (553 amino acids)

>TX73_024250 2-isopropylmalate synthase (Rhodopseudomonas palustris CGA009)
MLKNPETKYRPFVTPVDLPDRQWPSRRLRSAPRWLSTDLRDGNQSLANPMGVERKQRFYD
MLIAIGFKEIEVAFPSASQTEFDFVRSLINDDRIPNDVTIQVLTQSRTELIARTFESLEG
AQRAIVHLYNATAPLFRRVVFGMERRDVVDLAITGVTAMLDESLKRPGTDWTFEYSPETF
CFTEPDFALEICERVLDVWQPTPERPAILNLPATVEVAMPNVYADQIEWFCRNISRRDSV
VISLHPHNDRGTGVAAAELGLLAGADRVEGCLFGNGERTGNVDLVTLALNLYTQGIDPGL
SFPAMREVVNTVEHCTELPVHPRHPYAGEMVHAAFSGSHQDAIRKGFAAYAQRNDGLWEM
PYLPIDPADIGETYEAVIRVNSQSGKGGVAWVLEQDKGLKLPKRMQADFSTHVQKLADDL
GRELNTSDIWQCFAGCYLQRPTDRFVLVDYKETGPVGQRTFIGRIAIDGEVRSLSGRGNG
LISGVVAALQDAGGPALDVADYQEHAIGSGATAQAAAYVECRTSDGRTVFGTGIDPDVAT
ASVRALLHAANNA