Protein Info for TX73_023975 in Rhodopseudomonas palustris CGA009

Annotation: nitrogenase iron-molybdenum cofactor biosynthesis protein NifN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR01285: nitrogenase molybdenum-iron cofactor biosynthesis protein NifN" amino acids 2 to 430 (429 residues), 584.6 bits, see alignment E=6.3e-180 PF00148: Oxidored_nitro" amino acids 19 to 427 (409 residues), 387.2 bits, see alignment E=4.3e-120

Best Hits

Swiss-Prot: 61% identical to NIFN_BRADU: Nitrogenase iron-molybdenum cofactor biosynthesis protein NifN (nifN) from Bradyrhizobium diazoefficiens (strain JCM 10833 / IAM 13628 / NBRC 14792 / USDA 110)

KEGG orthology group: K02592, nitrogenase molybdenum-iron protein NifN (inferred from 100% identity to rpa:RPA4616)

Predicted SEED Role

"Nitrogenase FeMo-cofactor scaffold and assembly protein NifN" in subsystem Nitrogen fixation

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>TX73_023975 nitrogenase iron-molybdenum cofactor biosynthesis protein NifN (Rhodopseudomonas palustris CGA009)
MAQIVTSTKSCTVNPLRMSQPLGAALAFMGLRNCMPLLHGSQGCTSFGLVLFVRHFRESI
PLQTTAMSEVATVLGGFENVEQAIVNIVGRTKPDVIGICTTGVTEIKGDDLDGYIKMVRA
NHPELANVALVPVSTPDFKGAFEDGFAATVTRIVETLVETPAEGAAPDADRINVLAGSHL
TPGDIDELRDIIEAFGLVPTFLPDISGSLDGHVPDDFTPTTHGGVSVAEVVAMGGAGHTL
AFGEHMRKAAAALEAKAGVPFTLLSRVTGLAAADELMATLAKISGRPVPPKYRRQRSQLV
DAMLDGHFYFGGKSVAIGAEPDMLLNIGGWLADMGCTVSAAVTTTTSPSLAQVPSDEVLI
GDLEDLERRAEDCDLLVTHSHGRQAAERLSVPLFRMGLPMFDRLGAVHQVAVGYRGTRDL
IFAIGNLFIANIKEPDVNSWRSASACPDQTDAPAKAH